{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T11:26:55Z","timestamp":1765279615595},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,5,3]],"date-time":"2007-05-03T00:00:00Z","timestamp":1178150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold> This study evaluated whether diabetes modulates gene expression [interleukin (IL)\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20101ra, IL\u20106, IL\u20108, IL\u201010; tumor necrosis factor (TNF)\u2010<jats:italic>\u03b1<\/jats:italic>; interferon (IFN)\u2010<jats:italic>\u03b3<\/jats:italic>, receptor activator of NF\u2010<jats:italic>\u03ba<\/jats:italic>B ligand (RANKL) and osteoprotegerin (OPG)] in sites with periodontitis.<\/jats:p><jats:p><jats:bold>Materials and Methods:\u2002<\/jats:bold> Gingival biopsies were harvested and divided into three groups \u2013 Control group: systemically and periodontally healthy subjects (<jats:italic>n<\/jats:italic>\u2003=\u200310); Periodontitis group: systemically healthy subjects diagnosed with chronic periodontitis (<jats:italic>n<\/jats:italic>\u2003=\u200320); Diabetes group: type 1 diabetic subjects, diagnosed with chronic periodontitis (<jats:italic>n<\/jats:italic>\u2003=\u200320). Total RNA was obtained and analyzed by quantitative polymerase chain reaction.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Data analysis demonstrated that, except for OPG, mRNA levels for all factors were increased by inflammation (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). Interleukin\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20101ra, IL\u20106, IL\u20108, IFN\u2010<jats:italic>\u03b3<\/jats:italic>, and RANKL mRNA levels were higher in the diabetic group when compared with the control non\u2010periodontitis group (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05), whereas IL\u201010 and OPG were lower (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). No difference was observed for TNF\u2010<jats:italic>\u03b1<\/jats:italic> between diabetic and control groups (<jats:italic>P<\/jats:italic>\u2003&gt;\u20030.05). Diabetes lowered IL\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20108, IL\u201010, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, RANKL, and OPG mRNA levels in sites with comparable type of periodontitis (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). Moreover, increased RANKL:OPG and IL\u20106:IL\u201010 ratios were found.<\/jats:p><jats:p><jats:bold>Conclusion and clinical relevance:\u2002<\/jats:bold> Taken together, these data suggest that decreased levels of IL\u201010 and OPG may play an important role in the periodontal breakdown in diabetic patients.<\/jats:p>","DOI":"10.1111\/j.1601-0825.2006.01348.x","type":"journal-article","created":{"date-parts":[[2007,5,9]],"date-time":"2007-05-09T04:35:08Z","timestamp":1178685308000},"page":"594-599","source":"Crossref","is-referenced-by-count":43,"title":["Diabetes modulates gene expression in the gingival tissues of patients with chronic periodontitis"],"prefix":"10.1111","volume":"13","author":[{"given":"PM","family":"Duarte","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JBC","family":"Neto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MZ","family":"Casati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EA","family":"Sallum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"FH","family":"Nociti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00934.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1997.tb01835.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1998.69.2.113"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400406"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.3.260"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1983.54.12.714"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2003.10.005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8942"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2004.12.018"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345810600031101"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1981.52.4.167"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300216"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.22711.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-5491.2003.00953.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1997.tb01178.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1997.68.2.127"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.40"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1995.66.6.452"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1998.69.2.120"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80209-3"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1752-7325.2000.tb04072.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10763"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1381"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00821-0"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3597"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1601-0825.2006.01348.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1601-0825.2006.01348.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T17:13:21Z","timestamp":1697044401000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1601-0825.2006.01348.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,6]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,11]]}},"alternative-id":["10.1111\/j.1601-0825.2006.01348.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1601-0825.2006.01348.x","archive":["Portico"],"relation":{},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,6]]}}}