{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T22:06:40Z","timestamp":1698790000330},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,10,7]],"date-time":"2008-10-07T00:00:00Z","timestamp":1223337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:p><jats:bold>Objectives:\u2002<\/jats:bold> Tumor necrosis factor\u2010like weak inducer of apoptosis (TWEAK) is a multifunctional cytokine that has pro\u2010apoptotic, pro\u2010angiogenic and pro\u2010inflammatory effects. In liver, TWEAK leads to proliferation of progenitor oval cells, but not of mature hepatocytes. This study evaluated the hypothesis that TWEAK overexpression in salivary glands would lead to the proliferation of a salivary progenitor cell.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> A recombinant, serotype 5 adenoviral vector encoding human TWEAK, AdhTWEAK, was constructed, initially tested <jats:italic>in vitro<\/jats:italic>, and then administered to male Balb\/c mice via cannulation of Wharton\u2019s duct. TWEAK expression <jats:italic>in vivo<\/jats:italic> was monitored as protein secreted into saliva and serum by enzyme\u2010linked immunosorbent assays. Salivary cell proliferation was monitored by proliferating cell nuclear antigen staining and apoptosis was monitored using TUNEL staining.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> AdhTWEAK administration led to a dose\u2010dependent, transient TWEAK protein expression, detected primarily in saliva. Salivary epithelial cell proliferation was generalized, peaking on \u223cdays 2 and 3. TWEAK expression had no detectable effect on apoptosis of salivary epithelial cells.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Transient overexpression of TWEAK in murine salivary glands leads to a general proliferation of epithelial cells <jats:italic>vs<\/jats:italic> a selective stimulation of a salivary progenitor cell.<\/jats:p>","DOI":"10.1111\/j.1601-0825.2008.01474.x","type":"journal-article","created":{"date-parts":[[2008,10,7]],"date-time":"2008-10-07T07:28:15Z","timestamp":1223364495000},"page":"76-81","source":"Crossref","is-referenced-by-count":6,"title":["Transient TWEAK overexpression leads to a general salivary epithelial cell 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