{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T17:12:55Z","timestamp":1726161175444},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,9,8]],"date-time":"2011-09-08T00:00:00Z","timestamp":1315440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p><jats:italic>Oral Diseases<\/jats:italic> (2012) <jats:bold>18<\/jats:bold>, 132\u2013139<\/jats:p><jats:p><jats:bold>Objectives:\u2002<\/jats:bold> Autoantibodies from the sera of Sj\u00f6gren\u2019s syndrome patients (SS IgG) have been suggested to inhibit muscarinic receptor function. However, the acute nature of such an inhibitory effect remains controversial. In this study, we investigated the acute effects of SS IgG on muscarinic receptor function in human submandibular gland (HSG) cells.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> The effects of autoantibodies on muscarinic receptor function were studied using microspectrofluorimetry, whole\u2010cell patch clamp, immunofluorescence confocal microscopy, and a co\u2010immunoprecipitation assay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Carbachol (CCh) was found to consistently increase intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) and activate K<jats:sup>+<\/jats:sup> current in HSG cells. However, pretreatment of the cells with SS IgG for 5 or 30\u2003min significantly attenuated these responses, with a substantially more prominent effect after 30\u2003min of treatment. Like CCh, adenosine 5\u2032\u2010triphosphate (ATP) also increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and activated K<jats:sup>+<\/jats:sup> currents in HSG cells, although pretreatment with SS IgG did not affect the cellular response to ATP. CCh was found to reorganize \u03b1\u2010fodrin in HSG cells in a Ca<jats:sup>2+<\/jats:sup>\u2010dependent manner. However, pretreatment with SS IgG prevented the cytoskeletal reorganization of \u03b1\u2010fodrin induced by CCh.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> SS IgG acutely and reversibly inhibited muscarinic receptor function, thereby inhibiting the Ca<jats:sup>2+<\/jats:sup> mobilization necessary for the activation of K<jats:sup>+<\/jats:sup> currents and \u03b1\u2010fodrin reorganization in HSG cells.<\/jats:p>","DOI":"10.1111\/j.1601-0825.2011.01853.x","type":"journal-article","created":{"date-parts":[[2011,8,16]],"date-time":"2011-08-16T15:10:40Z","timestamp":1313507440000},"page":"132-139","source":"Crossref","is-referenced-by-count":12,"title":["Autoantibodies in Sj\u00f6gren\u2019s syndrome patients acutely inhibit muscarinic receptor function"],"prefix":"10.1111","volume":"18","author":[{"given":"M","family":"Jin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u2010M","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u2010Y","family":"Koo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u2010S","family":"Kho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u2010Y","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YW","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.42748.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar395"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21764"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20371"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.36057"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5312.604"},{"key":"e_1_2_9_8_1","first-page":"648","article-title":"Studies on rheumatoid factor: I. The effect of rheumatoid factor on the clearance of preformed immune complexes in mice","volume":"66","author":"Hogben DN","year":"1986","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509342227"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2220\/biomedres.29.131"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/ken064"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700173"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.2.C571"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.35.25121"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8942"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.67.041703.084745"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/clim.2002.5210"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.39.457"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(01)00047-4"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(90)90458-8"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/326498a0"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.116.1.127"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402871200"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-007-8003-8"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.61.6.554"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200007)43:7<1647::AID-ANR31>3.0.CO;2-P"},{"key":"e_1_2_9_27_1","first-page":"2157","article-title":"Prevalence of antibodies against alpha\u2010fodrin in Sjogren\u2019s syndrome: comparison of 2 sets of classification criteria","volume":"30","author":"Witte T","year":"2003","journal-title":"J Rheumatol"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1601-0825.2011.01853.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1601-0825.2011.01853.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T14:59:54Z","timestamp":1698677994000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1601-0825.2011.01853.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,8]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1111\/j.1601-0825.2011.01853.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1601-0825.2011.01853.x","archive":["Portico"],"relation":{},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,8]]}}}