{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:58:41Z","timestamp":1790510321133,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,10,24]],"date-time":"2011-10-24T00:00:00Z","timestamp":1319414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p><jats:italic>Oral Diseases<\/jats:italic>(2012)<jats:bold>18<\/jats:bold>, 169\u2013177<\/jats:p><jats:p><jats:bold>Objectives:\u2002<\/jats:bold>To isolate the CD133+CD44+ cells from human tongue squamous cell carcinoma (TSCC) Tca8113 cell line and investigate biological characteristics of them.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold>Immunomagnetic microbeads were applied to sort the CD133+CD44+ cells. Flow cytometry was used to detect isolation purity. The proliferation, clone\u2010formation efficiencies, invasion and migration, gene expressions, and tumor\u2010formation abilities were analyzed among CD133+CD44+, CD133\u2212CD44\u2212, and total population of cells.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>The average purities of CD133+ and CD44+ cells reached 97.3% and 98.7%, respectively. The proliferation of CD133+CD44+ cells was significantly higher than the other two groups. The clone\u2010forming efficiency of three groups was 70%, 8%, and 14%, respectively. The average invaded and migrated cell numbers of CD133+CD44+ and total population cells were 132 and 36.2, 311.6, and 156.2, respectively. The expressions of Bcl\u20102 and Sox2 in CD133+CD44+ cells were significantly higher than those in total population cells. A total of 10<jats:sup>4<\/jats:sup>CD133+CD44+ cells could form secondary tumors in nude mice, while the total population group needed 10<jats:sup>6<\/jats:sup>cells.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold>The CD133+CD44+ subpopulation cells possess stem\u2010like characteristics. They appear to be the potential targets for future biology therapy of human TSCC.<\/jats:p>","DOI":"10.1111\/j.1601-0825.2011.01860.x","type":"journal-article","created":{"date-parts":[[2011,9,29]],"date-time":"2011-09-29T16:02:08Z","timestamp":1317312128000},"page":"169-177","source":"Crossref","is-referenced-by-count":21,"title":["Biological characteristics of a cell subpopulation in tongue squamous cell 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