{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:21:55Z","timestamp":1793294515900,"version":"4.5.0"},"reference-count":64,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T00:00:00Z","timestamp":1332460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p><jats:italic>Oral Diseases<\/jats:italic> (2012) <jats:bold>18,<\/jats:bold> 602\u2013612<\/jats:p><jats:p><jats:bold>Objective:\u2002<\/jats:bold> Infection has been hypothesized as a contributing factor to bisphosphonate (BP)\u2010related osteonecrosis of the jaw (BRONJ). The objective of this study was to determine the bacterial colonization of jawbone and identify the bacterial phylotypes associated with BRONJ.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold> Culture\u2010independent 16S rRNA gene\u2010based molecular techniques were used to determine and compare the total bacterial diversity in bone samples collected from 12 patients with cancer (six, BRONJ with history of BP; six, controls without BRONJ, no history of BP but have infection).<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Denaturing gradient gel electrophoresis profile and Dice coefficient displayed a statistically significant clustering of profiles, indicating different bacterial population in BRONJ subjects and control. The top three genera ranked among the BRONJ group were <jats:italic>Streptococcus<\/jats:italic> (29%), <jats:italic>Eubacterium<\/jats:italic> (9%), and <jats:italic>Pseudoramibacter<\/jats:italic> (8%), while in the control group were <jats:italic>Parvimonas<\/jats:italic> (17%), <jats:italic>Streptococcus<\/jats:italic> (15%), and <jats:italic>Fusobacterium<\/jats:italic> (15%). H&amp;E sections of BRONJ bone revealed layers of bacteria along the surfaces and often are packed into the scalloped edges of the bone.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> This study using limited sample size indicated that the jawbone associated with BRONJ was heavily colonized by specific oral bacteria and there were apparent differences between the microbiota of BRONJ and controls.<\/jats:p>","DOI":"10.1111\/j.1601-0825.2012.01916.x","type":"journal-article","created":{"date-parts":[[2012,2,22]],"date-time":"2012-02-22T19:43:56Z","timestamp":1329939836000},"page":"602-612","source":"Crossref","is-referenced-by-count":56,"title":["Molecular profiling of oral microbiota in jawbone samples of bisphosphonate\u2010related osteonecrosis of the 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