{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T21:56:06Z","timestamp":1765230966746},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,5,30]],"date-time":"2012-05-30T00:00:00Z","timestamp":1338336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p><jats:italic>Oral Diseases<\/jats:italic> (2012) <jats:bold>18<\/jats:bold>, 771\u2013777<\/jats:p><jats:p><jats:bold>Objectives:\u2002<\/jats:bold> To characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold> We investigated the fungal populations on the tongue dorsum in 291 institutionalized elderly adults by molecular PCR\u2010based techniques using internal transcribed spacer regions of nuclear ribosomal DNA.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Quantitative PCR analysis showed that fungi were present on the tongue dorsum of 128 subjects at \u226510<jats:sup>4<\/jats:sup>\u2003CFU per sample, and 35 of them exceeded 10<jats:sup>5<\/jats:sup>\u2003CFU per sample. Length heterogeneity\u2010PCR analysis and nucleotide sequence determinations showed that Candida albicans was most frequently detected in those subjects with fungi at \u226510<jats:sup>4<\/jats:sup>\u2003CFU per sample (105 subjects), followed by Candida dubliniensis (78), Malassezia restricta (57), and Candida tropicalis (45). Statistical analysis revealed that those subjects with \u226510<jats:sup>5<\/jats:sup>\u2003CFU of fungi other than C.\u00a0albicans per sample had an increased risk of fever (\u22657 febrile days per 12\u2003months) compared with subjects with &lt;10<jats:sup>5<\/jats:sup>\u2003CFU per sample, after adjustment for other fever\u2010associated confounding factors.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> These data demonstrate that the oral cavity of the elderly is inhabited by a diverse array of fungi not limited to typical Candida species and they suggest that the diversity in distribution is associated with health status.<\/jats:p>","DOI":"10.1111\/j.1601-0825.2012.01944.x","type":"journal-article","created":{"date-parts":[[2012,4,27]],"date-time":"2012-04-27T15:26:45Z","timestamp":1335540405000},"page":"771-777","source":"Crossref","is-referenced-by-count":9,"title":["Molecular characterization of fungal populations on the tongue dorsum of institutionalized 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