{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:58:45Z","timestamp":1790218725152,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,2,13]],"date-time":"2007-02-13T00:00:00Z","timestamp":1171324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Congenital Anomalies"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:p><jats:bold>ABSTRACT\u2003<\/jats:bold>To evaluate the role of cobalamin (Cbl) on spermatogenesis, the effect of dietary vitamin B<jats:sub>12<\/jats:sub>deficiency on early spermatogenesis was histologically investigated in male fetuses and newborns in the first filial generation (F<jats:sub>1<\/jats:sub>males) of rats. There was no difference in the number of gonocytes and supporting cells of Sertoli in the gonad in male fetuses on day\u200316 of gestation and in the testes in F<jats:sub>1<\/jats:sub>males at 0\u2003days of age between vitamin B<jats:sub>12<\/jats:sub>\u2010deficient (VB12\u2010D) and vitamin B<jats:sub>12<\/jats:sub>\u2010supplemented (VB12\u2010S) groups. However, at 21\u2003days of age, a decreased number of spermatogonia and no spermatocytes were observed in the VB12\u2010D group. Numerous TUNEL positive cells were located among spermatocytes of the spermatogenic epithelium. The ultrastructural features examined using transmission electron microscopy were considered to be indicative of apoptosis. The incidence of seminiferous tubules having apoptotic cells was 51.5% in the VB12\u2010D group. At 60\u2003days of age, aplasia of the spermatids and spermatozoa was detected in the VB12\u2010D group. In the connective tissue between the seminiferous tubules, many interstitial Leydig cells and blood vessels were observed in the VB12\u2010D group, as compared with the VB12\u2010S group. These changes produced by vitamin B<jats:sub>12<\/jats:sub>deficiency can be reversed by providing a VB12\u2010S diet after weaning at 21\u2003days of age. From these findings, such a vitamin B<jats:sub>12<\/jats:sub>deficiency during gestation and lactation could affect the germ cells and especially damage spermatocytes in F<jats:sub>1<\/jats:sub>male rats, which indicates that Cbl may be an essential constituent in the meiosis of spermatogenesis.<\/jats:p>","DOI":"10.1111\/j.1741-4520.2006.00135.x","type":"journal-article","created":{"date-parts":[[2007,2,13]],"date-time":"2007-02-13T23:51:19Z","timestamp":1171410679000},"page":"9-15","source":"Crossref","is-referenced-by-count":23,"title":["Maternal vitamin B<sub>12<\/sub>deficiency affects spermatogenesis at the embryonic and immature stages in rats"],"prefix":"10.1111","volume":"47","author":[{"given":"Toshiaki","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhei","family":"Ebara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiko","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiko","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigenobu","family":"Kasai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihisa","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,2,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1002\/j.1939-4640.1998.tb02006.x","article-title":"Vitamin E deficiency causes incomplete spermatogenesis and affects the structural differentiation of epithelial cells of the epididymis in the rat","volume":"19","author":"Bensoussan K","year":"1988","journal-title":"J Androl"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(68)90044-5"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(91)90182-Z"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/S0015-0282(16)34271-6","article-title":"The treatment of oligospermia with stilbestrol and vitamin B","volume":"12","author":"Goodhope CD","year":"1961","journal-title":"Fertil Steril"},{"key":"e_1_2_6_6_1","first-page":"371","article-title":"Investigation and treatment of the infertile male","volume":"66","author":"Halim A","year":"1973","journal-title":"Proc Roy Soc Med"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0021932000010701"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0880051"},{"key":"e_1_2_6_9_1","first-page":"163","volume-title":"Developmental Nutrition","author":"Hurley LS","year":"1980"},{"key":"e_1_2_6_10_1","first-page":"581","article-title":"Clinical experience of methylcobalamin (CH3\u2010B12) for male infertility","volume":"30","author":"Isoyama R","year":"1984","journal-title":"Hinyokika kiyo"},{"key":"e_1_2_6_11_1","first-page":"80","article-title":"Effect of long term methylcobalamin therapy on idiopathic male infertility","volume":"32","author":"Iwamoto T","year":"1987","journal-title":"Jpn J Fertil Steril"},{"key":"e_1_2_6_12_1","first-page":"119","article-title":"Result of long\u2010term methylcobalamin treatment for male infertility","volume":"48","author":"Iwasaki A","year":"2003","journal-title":"Jpn J Fertil Steril"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.38.305"},{"key":"e_1_2_6_14_1","first-page":"17","article-title":"Effect of vitamin B12\u2010deficiency on testicular tissue in rats fed by pair\u2010feeding","volume":"67","author":"Kawata T","year":"1997","journal-title":"Int J Vitam Nutr Res"},{"key":"e_1_2_6_15_1","first-page":"18","article-title":"Effects of methylcobalamin on sperm counts and sperm motility in oligozoospermic cases","volume":"2","author":"Kimura M","year":"1981","journal-title":"J Androl"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02284263"},{"key":"e_1_2_6_17_1","first-page":"151","article-title":"Studies on the usefulness of a long\u2010term, high\u2010dose treatment of methylcobalamin for patients with oligozoospermia","volume":"33","author":"Moriyama H","year":"1987","journal-title":"Hinyokika kiyo"},{"key":"e_1_2_6_18_1","first-page":"243","article-title":"The treatment of male infertility. 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