{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:28:48Z","timestamp":1760297328621},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,12,21]],"date-time":"2004-12-21T00:00:00Z","timestamp":1103587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p>The effect of gadolinium ions on the sarcoplasmic reticulum (SR) calcium release channel\/ryanodine receptor (RyR1) was studied using heavy SR (HSR) vesicles and RyR1 isolated from rabbit fast twitch muscle. In the [<jats:sup>3<\/jats:sup>H]ryanodine binding assay, 5\u2003\u00b5<jats:sc>m<\/jats:sc> Gd<jats:sup>3+<\/jats:sup> increased the <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> of the [<jats:sup>3<\/jats:sup>H]ryanodine binding of the vesicles from 33.8\u2003n<jats:sc>m<\/jats:sc> to 45.6\u2003n<jats:sc>m<\/jats:sc> while <jats:italic>B<\/jats:italic><jats:sub>max<\/jats:sub>, referring to the binding capacity, was not affected significantly. In the presence of 18\u2003n<jats:sc>m<\/jats:sc>[<jats:sup>3<\/jats:sup>H]ryanodine and 100\u2003\u00b5<jats:sc>m<\/jats:sc> free Ca<jats:sup>2+<\/jats:sup>, Gd<jats:sup>3+<\/jats:sup> inhibited the binding of the radiolabeled ryanodine with an apparent <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> value of 14.7\u2003\u00b5<jats:sc>m<\/jats:sc> and a Hill coefficient of 3.17. In <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> experiments the time constant of <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> efflux from HSR vesicles increased from 90.9\u2003(\u00b1\u200311.1)\u2003ms to 187.7\u2003(\u00b1\u200324.9)\u2003ms in the presence of 20\u2003\u00b5<jats:sc>m<\/jats:sc> gadolinium. In single channel experiments gadolinium inhibited the channel activity from both the cytoplasmic (<jats:italic>cis<\/jats:italic>) (IC<jats:sub>50<\/jats:sub>\u2003=\u20035.65\u2003\u00b1\u20030.33\u2003\u00b5<jats:sc>m<\/jats:sc>, n<jats:sub>Hill<\/jats:sub>\u2003=\u20034.71) and the luminal (<jats:italic>trans<\/jats:italic>) side (IC<jats:sub>50<\/jats:sub>\u2003=\u20035.47\u2003\u00b1\u20030.24\u2003\u00b5<jats:sc>m<\/jats:sc>, n<jats:sub>Hill<\/jats:sub>\u2003=\u20034.31). The degree of inhibition on the <jats:italic>cis<\/jats:italic> side didn't show calcium dependency in the 100\u2003\u00b5<jats:sc>m<\/jats:sc> to 1\u2003m<jats:sc>m<\/jats:sc> Ca<jats:sup>2+<\/jats:sup> concentration range which indicates no competition with calcium on its regulatory binding sites. When Gd<jats:sup>3+<\/jats:sup> was applied at the <jats:italic>trans<\/jats:italic> side, EGTA was present at the <jats:italic>cis<\/jats:italic> side to prevent the binding of Gd<jats:sup>+3<\/jats:sup> to the cytoplasmic calcium binding regulatory sites of the RyR1 if Gd<jats:sup>3+<\/jats:sup> accidentally passed through the channel. The inhibition of the channel did not show any voltage dependence, which would be the case if Gd<jats:sup>3+<\/jats:sup> exerted its effect after getting to the <jats:italic>cis<\/jats:italic> side. Our results suggest the presence of inhibitory binding sites for Gd<jats:sup>3+<\/jats:sup> on both sides of the RyR1 with similar Hill coefficients and IC<jats:sub>50<\/jats:sub> values.<\/jats:p>","DOI":"10.1111\/j.1742-4658.2004.04486.x","type":"journal-article","created":{"date-parts":[[2005,1,12]],"date-time":"2005-01-12T14:01:36Z","timestamp":1105538496000},"page":"464-471","source":"Crossref","is-referenced-by-count":17,"title":["Effect of gadolinium on the ryanodine receptor\/sarcoplasmic reticulum calcium release channel of skeletal muscle"],"prefix":"10.1111","volume":"272","author":[{"given":"S\u00e1ndor","family":"S\u00e1rk\u00f6zi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Csaba","family":"Szegedi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bal\u00e1zs","family":"Luk\u00e1cs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Ronjat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Istv\u00e1n","family":"J\u00f3na","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,12,21]]},"reference":[{"key":"e_1_2_15_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01945959"},{"key":"e_1_2_15_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00790a012"},{"key":"e_1_2_15_4_2","first-page":"69P","article-title":"Gadolinium specifically blocks a component of voltage sensitive calcium current in neuroblastoma x glioma hybrid cells","volume":"381","author":"Docherty RJ","year":"1988","journal-title":"J Physiol"},{"key":"e_1_2_15_5_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017027"},{"key":"e_1_2_15_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.259.3.C515"},{"key":"e_1_2_15_7_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2340363"},{"key":"e_1_2_15_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(95)79880-0"},{"key":"e_1_2_15_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2238"},{"key":"e_1_2_15_10_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005630321863"},{"key":"e_1_2_15_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38666-1"},{"key":"e_1_2_15_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(90)82563-7"},{"key":"e_1_2_15_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2459777"},{"key":"e_1_2_15_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00238004"},{"key":"e_1_2_15_15_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.5.C1235"},{"key":"e_1_2_15_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(96)79831-4"},{"key":"e_1_2_15_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050117"},{"key":"e_1_2_15_18_2","first-page":"40P","article-title":"Effect of gadolinium on the ryanodine receptor\u2010calcium release channel","volume":"526","author":"Sarkozi S","year":"2000","journal-title":"J Physiol"},{"key":"e_1_2_15_19_2","first-page":"37, 56","volume-title":"Inorganic Complexes","author":"Martell AE","year":"1974"},{"key":"e_1_2_15_20_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005630321863"},{"key":"e_1_2_15_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-5754-4_10"},{"key":"e_1_2_15_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_15_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(88)57093-3"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1742-4658.2004.04486.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1742-4658.2004.04486.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T22:52:21Z","timestamp":1698792741000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1742-4658.2004.04486.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12,21]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1111\/j.1742-4658.2004.04486.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1742-4658.2004.04486.x","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12,21]]}}}