{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:12:08Z","timestamp":1791155528866,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2005,6,24]],"date-time":"2005-06-24T00:00:00Z","timestamp":1119571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:p>Bilitranslocase is a rat liver plasma membrane carrier, displaying a high\u2010affinity binding site for bilirubin. It is competitively inhibited by grape anthocyanins, including aglycones and their mono\u2010 and di\u2010glycosylated derivatives. In plant cells, anthocyanins are synthesized in the cytoplasm and then translocated into the central vacuole, by mechanisms yet to be fully characterized. The aim of this work was to determine whether a homologue of rat liver bilitranslocase is expressed in carnation petals, where it might play a role in the membrane transport of anthocyanins. The bromosulfophthalein\u2010based assay of rat liver bilitranslocase transport activity was implemented in subcellular membrane fractions, leading to the identification of a bromosulfophthalein carrier (<jats:italic>K<\/jats:italic><jats:sub>M<\/jats:sub>\u2003=\u20035.3\u2003\u00b5<jats:sc>m<\/jats:sc>), which is competitively inhibited by cyanidine 3\u2010glucoside (<jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub>\u2003=\u200351.6\u2003\u00b5<jats:sc>m<\/jats:sc>) and mainly noncompetitively by cyanidin (<jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub>\u2003=\u200388.3\u2003\u00b5<jats:sc>m<\/jats:sc>). Two antisequence antibodies against bilitranslocase inhibited this carrier. In analogy to liver bilitranslocase, one antibody identified a bilirubin\u2010binding site (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>\u2003=\u20031.7\u2003n<jats:sc>m<\/jats:sc>) in the carnation carrier. The other antibody identified a high\u2010affinity binding site for cyanidine 3\u2010glucoside (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>\u2003=\u20031.7\u2003\u00b5<jats:sc>m<\/jats:sc>) on the carnation carrier only, and a high\u2010affinity bilirubin\u2010binding site (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>\u2003=\u20030.33\u2003n<jats:sc>m<\/jats:sc>) on the liver carrier only. Immunoblots showed a putative homologue of rat liver bilitranslocase in both plasma membrane and tonoplast fractions, isolated from carnation petals. Furthermore, only epidermal cells were immunolabelled in petal sections examined by microscopy. In conclusion, carnation petals express a homologue of rat liver bilitranslocase, with a putative function in the membrane transport of secondary metabolites.<\/jats:p>","DOI":"10.1111\/j.1742-4658.2005.04751.x","type":"journal-article","created":{"date-parts":[[2005,6,24]],"date-time":"2005-06-24T17:49:51Z","timestamp":1119635391000},"page":"3282-3296","source":"Crossref","is-referenced-by-count":34,"title":["Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase"],"prefix":"10.1111","volume":"272","author":[{"given":"Sabina","family":"Passamonti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Cocolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Braidot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Petrussa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Peresson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nevenka","family":"Medic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Macri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Vianello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,24]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5266(02)00256-X"},{"key":"e_1_2_7_3_2","first-page":"673","article-title":"The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer","volume":"52","author":"Middleton E","year":"2000","journal-title":"Pharmacol Rev"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.43.060192.001325"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00312.x"},{"key":"e_1_2_7_6_2","first-page":"119","article-title":"Compartmentation of plant phenolic compounds; 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