{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:14:37Z","timestamp":1777497277706,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2005,7,22]],"date-time":"2005-07-22T00:00:00Z","timestamp":1121990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:p> <jats:italic>Trypanosoma cruzi<\/jats:italic>, the parasitic protozoan that causes Chagas disease, contains a major cysteine proteinase, cruzipain. This lysosomal enzyme bears an unusual C\u2010terminal extension that contains a number of post\u2010translational modifications, and most antibodies in natural and experimental infections are directed against it. In this report we took advantage of UV\u2010MALDI\u2010TOF mass spectrometry in conjunction with peptide N\u2010glycosidase F deglycosylation and high performance anion exchange chromatography analysis to address the structure of the N\u2010linked oligosaccharides present in this domain. The UV\u2010MALDI\u2010TOF MS analysis in the negative\u2010ion mode, using nor\u2010harmane as matrix, allowed us to determine a new striking feature in cruzipain: sulfated high\u2010mannose type oligosaccharides. Sulfated GlcNAc<jats:sub>2<\/jats:sub>Man<jats:sub>3<\/jats:sub> to GlcNAc<jats:sub>2<\/jats:sub>Man<jats:sub>9<\/jats:sub> species were identified. In accordance, after chemical or enzymatic desulfation, the corresponding signals disappeared. In addition, by UV\u2010MALDI\u2010TOF MS analysis (a) a main population of high\u2010mannose type oligosaccharides was shown in the positive\u2010ion mode, (b) lactosaminic glycans were also identified, among them, structures corresponding to monosialylated species were detected, and (c) as an interesting fact a fucosylated oligosaccharide was also detected. The presence of the deoxy sugar was further confirmed by high performance anion exchange chromatography. In conclusion, the total number of oligosaccharides occurring in cruzipain was shown to be much higher than previous estimates. This constitutes the first report on the presence of sulfated glycoproteins in Trypanosomatids.<\/jats:p>","DOI":"10.1111\/j.1742-4658.2005.04787.x","type":"journal-article","created":{"date-parts":[[2005,7,26]],"date-time":"2005-07-26T18:24:47Z","timestamp":1122402287000},"page":"3803-3815","source":"Crossref","is-referenced-by-count":44,"title":["Structural analysis of the N\u2010glycans of the major cysteine proteinase of <i>Trypanosoma cruzi<\/i>"],"prefix":"10.1111","volume":"272","author":[{"given":"Mariana","family":"Barboza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vilma 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