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In order to analyse the interactions between globin and linker subunits, dissociation and reassociation experiments were carried out under whereby <jats:italic>Arenicola<\/jats:italic> hexagonal bilayer haemoglobin was exposed to urea and alkaline pH and the effect was followed by gel filtration, SDS\/PAGE, UV\u2010visible spectrophotometry, electrospray\u2010ionization MS, multiangle laser light scattering and transmission electron microscopy. The analysis of <jats:italic>Arenicola<\/jats:italic> haemoglobin dissociation indicates a novel and complex mechanism of dissociation compared with other annelid extracellular haemoglobins studied to date. Even though the chemically induced dissociation triggers partial degradation of some subunits, spontaneous reassociation was observed, to some extent. Parallel dissociation of <jats:italic>Lumbricus<\/jats:italic> haemoglobin under similar conditions shows striking differences that allow us to propose a hypothesis on the nature of the intersubunit contacts that are essential to form and to hold such a complex quaternary structure.<\/jats:p>","DOI":"10.1111\/j.1742-4658.2006.05151.x","type":"journal-article","created":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T08:11:16Z","timestamp":1140682276000},"page":"1582-1596","source":"Crossref","is-referenced-by-count":29,"title":["Novel dissociation mechanism of a polychaetous annelid extracellular haemoglobin"],"prefix":"10.1111","volume":"273","author":[{"given":"Morgane","family":"Rousselot","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Le Guen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Chabasse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Zal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,3,8]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(91)90165-A"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr9600058"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.13.7107"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.85_1a.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2825"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.47.30007"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.40.28206"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00803"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2001.81.2.569"},{"key":"e_1_2_7_11_2","first-page":"145","article-title":"Dissociation of the extracellular hemoglobin of Arenicola marina","volume":"65","author":"Vinogradov SN","year":"1979","journal-title":"Comp Biochem Physiol"},{"key":"e_1_2_7_12_2","first-page":"1419","article-title":"[Recent Research on the Electron Microscopic Study of the Hemoglobin (Erythrocruorine) of Arenicola Marina L. & Its Dissociation.]","volume":"157","author":"Roche J","year":"1963","journal-title":"C R Seances Soc Biol Fil"},{"key":"e_1_2_7_13_2","volume-title":"no. 00\/07031. 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