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To test this hypothesis we analysed the glycosphingolipid sulfatide, which is highly abundant in myelin and has mostly long fatty acids. We used a negative ion electrospray tandem mass spectrometry precursor ion scan to analyse the molecular species of sulfatide in cultured astrocytes and a mouse model of the human disease metachromatic leukodystrophy. In these arylsulfatase\u2003A (ASA)\u2010deficient mice sulfatide accumulates intracellularly in neurons and astrocytes. Immunocytochemistry was also performed on cultured astrocytes and analysed using confocal laser scanning microscopy. Analyses of the molecular species showed that cultured astrocytes contained sulfatide with a predominance of stearic acid (C18), which was located in large intracellular vesicles throughout the cell body and along the processes. The same was seen in ASA\u2010deficient mice, which accumulated a higher proportion (15\u2003mol% compared with 8\u2003mol% in control mice) of sulfatide with stearic acid. We conclude that the major fatty acid composition of sulfatide differs between white and grey matter, with neurons and astrocytes containing mostly short\u2010chain fatty acids with an emphasis on stearic acid. Based on our results, we speculate that the fatty acid chain length of sulfatide might determine its intracellular (short chain) or extracellular (long chain) location and thereby its functions.<\/jats:p>","DOI":"10.1111\/j.1742-4658.2006.05195.x","type":"journal-article","created":{"date-parts":[[2006,3,24]],"date-time":"2006-03-24T10:38:48Z","timestamp":1143196728000},"page":"1782-1790","source":"Crossref","is-referenced-by-count":42,"title":["Sulfatide with short fatty acid dominates in astrocytes and 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