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Using mass spectrometry and western blotting, we have now analyzed the peptide <jats:italic>N<\/jats:italic>\u2010glycosidase A\u2010released N\u2010glycans of adults of this species. The presence of hybrid bi\u2010 and triantennary N\u2010glycans, some modified by core \u03b11,6\u2010fucose and peripheral phosphorylcholine, was demonstrated by LC\/electrospray ionization (ESI)\u2010Q\u2010TOF\u2010MS\/MS, as was the presence of paucimannosidic N\u2010glycans, some of which carry core \u03b11,3\u2010fucose, and oligomannosidic oligosaccharides. Western blotting verified the presence of protein\u2010bound phosphorylcholine and core \u03b11,3\u2010fucose, whereas glycosyltransferase assays showed the presence of core \u03b11,6\u2010fucosyltransferase and Lewis\u2010type \u03b11,3\u2010fucosyltransferase activities. Although, the unusual tri\u2010 and tetrafucosylated glycans found in the model nematode <jats:italic>Caenorhabditis elegans<\/jats:italic> were not found, the vast majority of the N\u2010glycans found in <jats:italic>A.\u2003suum<\/jats:italic> represent a subset of those found in <jats:italic>C.\u2003elegans<\/jats:italic>; thus, our data demonstrate that the latter is an interesting glycobiological model for parasitic nematodes.<\/jats:p>","DOI":"10.1111\/j.1742-4658.2006.05615.x","type":"journal-article","created":{"date-parts":[[2006,12,21]],"date-time":"2006-12-21T05:58:52Z","timestamp":1166680732000},"page":"714-726","source":"Crossref","is-referenced-by-count":56,"title":["N\u2010Glycans of the porcine nematode parasite <i>Ascaris suum<\/i> are modified with phosphorylcholine and core fucose residues"],"prefix":"10.1111","volume":"274","author":[{"given":"Gerald","family":"P\u00f6ltl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Kerner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Paschinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain B. 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