{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:27:47Z","timestamp":1791325667825,"version":"4.3.1"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,4,26]],"date-time":"2010-04-26T00:00:00Z","timestamp":1272240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p>Atrial natriuretic peptide (ANP), via its guanylyl cyclase\u2003A (GC\u2010A) receptor and intracellular guanosine 3\u2032,5\u2032\u2010cyclic monophosphate production, is critically involved in the regulation of blood pressure. In patients with chronic heart failure, the plasma levels of ANP are increased, but the cardiovascular actions are severely blunted, indicating a receptor or postreceptor defect. Studies on metabolically labelled GC\u2010A\u2010overexpressing cells have indicated that GC\u2010A is extensively phosphorylated, and that ANP\u2010induced homologous desensitization of GC\u2010A correlates with receptor dephosphorylation, a mechanism which might contribute to a loss of function <jats:italic>in\u2003vivo<\/jats:italic>. In this study, tandem MS analysis of the GC\u2010A receptor, expressed in the human embryonic kidney cell line HEK293, revealed unambiguously that the intracellular domain of the receptor is phosphorylated at multiple residues: Ser487, Ser497, Thr500, Ser502, Ser506, Ser510 and Thr513. MS quantification based on multiple reaction monitoring demonstrated that ANP\u2010provoked desensitization was accompanied by a complex pattern of receptor phosphorylation and dephosphorylation. The population of completely phosphorylated GC\u2010A was diminished. However, intriguingly, the phosphorylation of GC\u2010A at Ser487 was selectively enhanced after exposure to ANP. The functional relevance of this observation was analysed by site\u2010directed mutagenesis. The substitution of Ser487 by glutamate (which mimics phosphorylation) blunted the activation of the GC\u2010A receptor by ANP, but prevented further desensitization. Our data corroborate previous studies suggesting that the responsiveness of GC\u2010A to ANP is regulated by phosphorylation. However, in addition to the dephosphorylation of the previously postulated sites (Ser497, Thr500, Ser502, Ser506, Ser510), homologous desensitization seems to involve the phosphorylation of GC\u2010A at Ser487, a newly identified site of phosphorylation. The identification and further characterization of the specific mechanisms involved in the downregulation of GC\u2010A responsiveness to ANP may have important pathophysiological implications.<\/jats:p><jats:p><jats:bold>Structured digital abstract<\/jats:bold><\/jats:p><jats:p><jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/mint.bio.uniroma2.it\/mint\/search\/interaction.do?interactionAc=MINT-7713870\">MINT\u20107713870<\/jats:ext-link>, <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/mint.bio.uniroma2.it\/mint\/search\/interaction.do?interactionAc=MINT-7713887\">MINT\u20107713887<\/jats:ext-link>: <jats:italic>PMCA<\/jats:italic> (uniprotkb:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.uniprot.org\/uniprot\/P20020\">P20020<\/jats:ext-link>) and <jats:italic>GC\u2010A<\/jats:italic> (uniprotkb:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.uniprot.org\/uniprot\/P18910\">P18910<\/jats:ext-link>) <jats:italic>colocalize<\/jats:italic> (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ebi.ac.uk\/ontology-lookup\/?termId=MI:0403\">MI:0403<\/jats:ext-link>) by <jats:italic>fluorescence microscopy<\/jats:italic> (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ebi.ac.uk\/ontology-lookup\/?termId=MI:0416\">MI:0416<\/jats:ext-link>)<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p>","DOI":"10.1111\/j.1742-4658.2010.07658.x","type":"journal-article","created":{"date-parts":[[2010,4,26]],"date-time":"2010-04-26T12:38:22Z","timestamp":1272285502000},"page":"2440-2453","source":"Crossref","is-referenced-by-count":37,"title":["Homologous desensitization of guanylyl cyclase\u2003A, the receptor for atrial natriuretic peptide, is associated with a complex phosphorylation pattern"],"prefix":"10.1111","volume":"277","author":[{"given":"Juliane","family":"Schr\u00f6ter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9 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