{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:13:53Z","timestamp":1790662433373,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:p>The mechanisms whereby hepatic fibrosis develops in chronic liver diseases remain incompletely defined. Here, we sought to examine whether microRNA (miRNA) became dysregulated in dimethylnitrosamine\u2010induced hepatic fibrosis in rats. Our microarray analysis revealed that the miR\u201034 family was upregulated along with other miRNAs in liver fibrotic tissues. Six miRNAs, such as rno\u2010miR\u2010878, were downregulated. The findings were confirmed by RT\u2010PCR assays. Gene ontology analysis further showed that many of these dysregulated miRNAs were involved in lipid\/fatty acid metabolism. The acyl\u2010CoA synthetase long\u2010chain family member\u20031 (ACSL1) gene contained specific binding sites for miR\u201034a\/miR\u201034c. Additional enhanced green fluorescence protein reporter activity assays indicated that the miR\u201034 family targeted <jats:italic>ACSL1<\/jats:italic>. Our RT\u2010PCR and immunoblotting assays further demonstrated that both the mRNA and protein levels of ACSL1 were markedly reduced in fibrotic liver tissues. Our findings suggest that miRNA becomes dysregulated during hepatic fibrosis, and that the miR\u201034 family may be involved in the process by targeting <jats:italic>ACSL1<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1742-4658.2011.08075.x","type":"journal-article","created":{"date-parts":[[2011,3,3]],"date-time":"2011-03-03T03:53:52Z","timestamp":1299124432000},"page":"1522-1532","source":"Crossref","is-referenced-by-count":100,"title":["The rno\u2010miR\u201034 family is upregulated and targets <i>ACSL1<\/i> in dimethylnitrosamine\u2010induced hepatic fibrosis in 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