{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T04:14:33Z","timestamp":1778991273128,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2011,6,13]],"date-time":"2011-06-13T00:00:00Z","timestamp":1307923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:p><jats:italic>Pasteurella\u2003multocida<\/jats:italic> toxin (PMT) is a virulence factor responsible for the pathogenesis of some <jats:italic>Pasteurellosis<\/jats:italic>. PMT exerts its toxic effects through the activation of heterotrimeric GTPase (G<jats:sub>q<\/jats:sub>, G<jats:sub>12\/13<\/jats:sub> and G<jats:sub>i<\/jats:sub>)\u2010dependent pathways, by deamidating a glutamine residue in the \u03b1 subunit of these GTPases. However, the enzymatic characteristics of PMT are yet to be analyzed in detail because the deamidation has only been observed in cell\u2010based assays. In the present study, we developed rat monoclonal antibodies, specifically recognizing the deamidated G\u03b1<jats:sub>q<\/jats:sub>, to detect the actions of PMT by immunological techniques such as western blotting. Using the monoclonal antibodies, we found that the toxin deamidated G\u03b1<jats:sub>q<\/jats:sub> only under reducing conditions. The C\u2010terminal region of PMT, C\u2010PMT, was more active than the full\u2010length PMT. The C3 domain possessing the enzyme core catalyzed the deamidation <jats:italic>in\u2003vitro<\/jats:italic> without any other domains. These results not only support previous observations on toxicity, but also provide insights into the enzymatic nature of PMT. In addition, we present several lines of evidence that G\u03b1<jats:sub>11<\/jats:sub>, as well as G\u03b1<jats:sub>q<\/jats:sub>, could be a substrate for PMT.<\/jats:p>","DOI":"10.1111\/j.1742-4658.2011.08197.x","type":"journal-article","created":{"date-parts":[[2011,5,27]],"date-time":"2011-05-27T17:23:01Z","timestamp":1306516981000},"page":"2702-2712","source":"Crossref","is-referenced-by-count":29,"title":["Enzymatic actions of <i>Pasteurella\u2003multocida<\/i> toxin detected by monoclonal antibodies recognizing the deamidated \u03b1 subunit of the heterotrimeric GTPase G<sub>q<\/sub>"],"prefix":"10.1111","volume":"278","author":[{"given":"Shigeki","family":"Kamitani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinpei","family":"Ao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirono","family":"Toshima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taro","family":"Tachibana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makiko","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kengo","family":"Kitadokoro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aya","family":"Fukui\u2010Miyazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Abe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiko","family":"Horiguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,6,13]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.10.4240"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.3.959-965.1996"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.1.123"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2005.01.002"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74039-3"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04264.x"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.2.1268"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405353200"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M507203200"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007819200"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.10.5640"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.1.439"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.3.2239"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)67725-2"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M803435200"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0900160106"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3628-3634.2001"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0272959"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7839-7850.2001"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5636-5642.1998"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.11.4909-4914.1993"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.9.3814"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608197104"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.102285"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0908700107"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1118890"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.21.11623"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1159\/000186689"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.23.9113"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.140210297"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M708919200"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309106030375"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304570200"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.15.4304"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1247\/csf.20.151"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02615077"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00630-9"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1742-4658.2011.08197.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1742-4658.2011.08197.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T11:58:41Z","timestamp":1697457521000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1742-4658.2011.08197.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6,13]]},"references-count":38,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2011,8]]}},"alternative-id":["10.1111\/j.1742-4658.2011.08197.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1742-4658.2011.08197.x","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,6,13]]}}}