{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T15:07:20Z","timestamp":1794236840349,"version":"4.9.0"},"reference-count":25,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2012,6,15]],"date-time":"2012-06-15T00:00:00Z","timestamp":1339718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:p>miRNAs play an important role in many human diseases, including cancer metastasis. However, the mechanisms by which miRNAs regulate intrahepatic cholangiocarcinoma metastasis remain poorly understood. In the present study, we assayed the expression level of miR\u2010214 in intrahepatic cholangiocarcinoma tissues by real\u2010time PCR, and defined the target gene and biological function by luciferase reporter assay and Western blot analysis. We found that the miR\u2010214 levels were remarkably decreased in metastatic intrahepatic cholangiocarcinoma tissues compared to non\u2010metastatic tissues. Inhibition of miR\u2010214 levels by its inhibitor promoted metastasis of human intrahepatic cholangiocarcinoma cell. We further demonstrated that down\u2010regulation of miR\u2010214 increased the transcript levels of the epithelial\u2013mesenchymal transition\u2010associated gene <jats:italic>Twist<\/jats:italic>, and then decreased E\u2010cadherin levels. We confirmed that down\u2010regulation of miR\u2010214 promoted the epithelial\u2013mesenchymal transition by directly targeting the <jats:italic>Twist<\/jats:italic> gene. These results suggest an important role for miR\u2010214 in regulating metastasis of intrahepatic cholangiocarcinoma, and potential application of miR\u2010214 in intrahepatic cholangiocarcinoma therapy.<\/jats:p>","DOI":"10.1111\/j.1742-4658.2012.08618.x","type":"journal-article","created":{"date-parts":[[2012,4,27]],"date-time":"2012-04-27T15:49:21Z","timestamp":1335541761000},"page":"2393-2398","source":"Crossref","is-referenced-by-count":114,"title":["Down\u2010regulation of miR\u2010214 contributes to intrahepatic cholangiocarcinoma metastasis by targeting Twist"],"prefix":"10.1111","volume":"279","author":[{"given":"Bin","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingqi","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,15]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.48.1.80"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-30-2"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.48.6.816"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/29.3.147"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209808"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-011-1023-z"},{"key":"e_1_2_6_8_2","first-page":"416","article-title":"TWIST expression in hypopharyngeal cancer and the mechanism of TWIST\u2010induced promotion of metastasis","volume":"27","author":"Yu L","year":"2012","journal-title":"Oncol Rep"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.364"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2009.140"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-2245"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013779"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00541-5"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000879"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2008.09.015"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-10-15"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1159\/000077991"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.5115\/acb.2010.43.2.110"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.10165"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.337642"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.252"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.102"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn920"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23660"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni178"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026340"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1742-4658.2012.08618.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1742-4658.2012.08618.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T17:42:11Z","timestamp":1696786931000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1742-4658.2012.08618.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,15]]},"references-count":25,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["10.1111\/j.1742-4658.2012.08618.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1742-4658.2012.08618.x","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,15]]}}}