{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:37:39Z","timestamp":1759963059952},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,9,7]],"date-time":"2012-09-07T00:00:00Z","timestamp":1346976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Wound Journal"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>Cultured dermal substitutes have been used for the treatment of chronic skin ulcers; however, the biological risks of animal\u2010derived materials in the culture process such as foetal bovine serum (FBS) have been reported. In this study, we prepared an autologous fibroblast\u2010seeded artificial dermis (AFD) using animal\u2010product\u2010free medium supplemented with 2% patient autologous serum and without any animal\u2010derived materials such as trypsin in the culturing process. We applied the AFD in five patients with diabetic ulcers and investigated its safety and efficacy. As the primary endpoint, we defined \u2018wound bed improvement' according to the percentage of granulation area to the whole wound area on day 21, and 60% or higher was regarded as improved. The mean age of the patients was 60\u00b76 years and the mean duration of the ulcer was 22\u00b76 months. In the evaluation of the primary endpoint, the \u2018wound bed\u2019 was improved in all patients [proportion of improvement: 100%, 95% confidence interval (CI): 48% to 100%]. Three patients had complete wound healing within 12 weeks after application and two patients had &gt;80% wound healing at 12 weeks. Side effects were not serious. Our AFD may be a safe and effective treatment of diabetic ulcers.<\/jats:p>","DOI":"10.1111\/j.1742-481x.2012.01064.x","type":"journal-article","created":{"date-parts":[[2012,9,7]],"date-time":"2012-09-07T13:37:16Z","timestamp":1347025036000},"page":"183-189","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["An exploratory clinical study on the safety and efficacy of an autologous fibroblast\u2010seeded artificial skin cultured with animal product\u2010free medium in patients with diabetic foot 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