{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T07:22:15Z","timestamp":1775028135332,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,12,8]],"date-time":"2011-12-08T00:00:00Z","timestamp":1323302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Immunogenetics"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Tuberculosis (TB) is one of most important chronic infectious diseases caused by <jats:italic>Mycobacterium tuberculosis<\/jats:italic> and remains a major global health problem. In the study, we developed the DNA vaccine encoding fusion protein of antigen 85\u2003A and 6\u2003kDa early secretory antigen target of <jats:italic>M.\u00a0tuberculosis<\/jats:italic> as well as the cytokine IL\u201021 to investigate its immune protective efficacy against <jats:italic>M.\u00a0tuberculosis<\/jats:italic> challenge in mice after the DNA vaccine priming and Bacille Calmette\u2010Gu\u00e9rin (BCG) boosting. Compared with the different control groups, the intranasal DNA vaccine priming twice and BCG boosting once markedly increased the cytotoxicities of natural killer cells and splenocytes and enhanced the interferon\u2010\u03b3 level in the splenocyte supernatant as well as sIgA level in bronchoalveolar lavage in the vaccinated mice. Importantly, this heterologous prime\u2013boost strategy significantly decreased the bacterial load in the mouse lungs in contrast to that of intranasal or subcutaneous BCG immunization alone. These findings provide further approaches for mucosal\u2010targeted prime\u2013boost vaccination to fight against TB.<\/jats:p>","DOI":"10.1111\/j.1744-313x.2011.01066.x","type":"journal-article","created":{"date-parts":[[2011,12,8]],"date-time":"2011-12-08T14:31:19Z","timestamp":1323354679000},"page":"183-190","source":"Crossref","is-referenced-by-count":28,"title":["Protection against <i>Mycobacterium tuberculosis<\/i> challenge in mice by DNA vaccine Ag85A\u2010ESAT\u20106\u2010IL\u201021 priming and BCG boosting"],"prefix":"10.1111","volume":"39","author":[{"given":"J.","family":"Dou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404347101"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0012.2000.140611.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01290-06"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(01)00162-4"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.9.6353"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02551.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/08820130701690741"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2009.04.002"},{"key":"e_1_2_6_10_1","first-page":"1214","article-title":"Global trends in resistance to antituberculosis drugs. World Health Organization\u2013International Union against tuberculosis and lung disease working group on anti\u2010tuberculosis drug resistance surveillance","volume":"344","author":"Espinal M.A.","year":"2001","journal-title":"The New England Journal of Medicine"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.11.087510"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903357"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.1.1.63"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2007.02031.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-10-51"},{"key":"e_1_2_6_16_1","first-page":"800","article-title":"Constructing nucleic acid vaccine expressing fusion antigens of Mycobacterium tuberculosis Ag85A and ESAT\u20106, and Interleukin 21 as well as studying its immune effect in mice","volume":"20","author":"Jinlong L.","year":"2009","journal-title":"Letters Biotechnology"},{"key":"e_1_2_6_17_1","first-page":"209","article-title":"Cellular response to gene expression profiles of different hepatitis C virus core proteins in the Huh\u20107 cell line with microarray analysis","volume":"5","author":"Jun D.","year":"2005","journal-title":"Journal of Nanoscience Nanotechnology"},{"key":"e_1_2_6_18_1","first-page":"1800","article-title":"Effect of hepatitis C virus core shadow protein expressed in human hepatoma cell line on human gene expression profiles","volume":"21","author":"Jun D.","year":"2006","journal-title":"Journal of Gastroenterology and Hepatology"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/08820130600634550"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.6.12.4960"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1221"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.249"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann T.R.","year":"1983","journal-title":"Journal of Immunological Methods"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00004-07"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1086\/341901"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1184784"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005531"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.6.4.10711"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568010054022123"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI6224"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.10.6357"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175194"}],"container-title":["International Journal of Immunogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1744-313X.2011.01066.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1744-313X.2011.01066.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T11:50:04Z","timestamp":1698666604000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1744-313X.2011.01066.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,8]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1111\/j.1744-313X.2011.01066.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1744-313x.2011.01066.x","archive":["Portico"],"relation":{},"ISSN":["1744-3121","1744-313X"],"issn-type":[{"value":"1744-3121","type":"print"},{"value":"1744-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,8]]}}}