{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T09:10:59Z","timestamp":1759482659619},"reference-count":10,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,6,9]],"date-time":"2011-06-09T00:00:00Z","timestamp":1307577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ther Apher Dial"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In two patients with steroid\u2010resistant nephrotic syndrome (SRNS), we investigated the relationship between clinical findings during immunosuppressive therapy and multiple drug resistant gene\u20101 (<jats:italic>MDR\u20101<\/jats:italic>) expression. <jats:italic>MDR\u20101<\/jats:italic> was detected by real\u2010time polymerase chain reaction (PCR). In a boy who initially developed SRNS at 3\u2003years, we observed <jats:italic>MDR\u20101<\/jats:italic> expression over 3\u2003years. Maximal and minimal <jats:italic>MDR\u20101<\/jats:italic> expression were 90\u2003000 and 7800 copies\/\u00b5g RNA, respectively. In a 4\u2010year\u2010old boy who initially developed SRNS at 3\u2003years, we determined <jats:italic>MDR\u20101<\/jats:italic> expression over 2\u2003years. Maximal and minimal <jats:italic>MDR\u20101<\/jats:italic> expression were 42\u2003000 and 6900, respectively. <jats:italic>MDR\u20101<\/jats:italic> evaluation requires determination of <jats:italic>MDR\u20101<\/jats:italic> expression at several time points in a clinical course. Establishment of a normal expression may be needed for each individual patient. Increasing <jats:italic>MDR\u20101<\/jats:italic> during remission was followed soon by recurrences, an observation that may be a guide for therapeutic choice. LDL influences a humoral factor involved in <jats:italic>MDR\u20101<\/jats:italic> expression. Both patients responded to LDL adsorption therapy because of elevated LDL levels. While cyclosporine A therapy gradually decreased <jats:italic>MDR\u20101<\/jats:italic> expression, LDL adsorption therapy decreased expression sharply. Based on the results of the present study, LDL adsorption therapy could contribute to the amelioration of drug sensitivity for immunosuppressants including corticosteroids via inhibitory effects on <jats:italic>MDR\u20101<\/jats:italic> expression.<\/jats:p>","DOI":"10.1111\/j.1744-9987.2011.00984.x","type":"journal-article","created":{"date-parts":[[2011,6,10]],"date-time":"2011-06-10T08:28:03Z","timestamp":1307694483000},"page":"499-503","source":"Crossref","is-referenced-by-count":6,"title":["Low\u2010Density Lipoprotein Adsorption Therapy Can Restore Drug Sensitivity for Immunosuppressants Via Inhibitory Effects Upon <i>MDR\u20101<\/i> Gene Expression"],"prefix":"10.1111","volume":"15","author":[{"given":"Satoshi","family":"Ueda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keisuke","family":"Sugimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidehiko","family":"Yanagida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinsuke","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoki","family":"Miyazawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Sakata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuru","family":"Okada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsukasa","family":"Takemura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,6,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(93)90095-Q"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1681\/ASN.V32254","article-title":"Severe hypercholesterolemia inhibits cyclosporin A efficacy in a dose\u2010dependent manner in children with nephritic syndrome","volume":"3","author":"Ingulli E","year":"1992","journal-title":"J Am Soc Nephrol"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.5414\/CNP67341"},{"key":"e_1_2_6_5_2","first-page":"6901","article-title":"Reversal by cefoperazone of resistance to etoposide, doxorubicin, and vinblastine in multidrug resistant human sarcoma cells","volume":"49","author":"Gosland MP","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200X.2008.02541.x"},{"key":"e_1_2_6_7_2","first-page":"218","article-title":"Resistance to therapy in primary nephrotic syndrome: effect of MDR1 gene activity","volume":"8","author":"Stachowski J","year":"2000","journal-title":"Pol Merkur Lekarski"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/00498250903226025"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-004-1569-y"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2004.00803.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-006-0275-3"}],"container-title":["Therapeutic Apheresis and Dialysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1744-9987.2011.00984.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1744-9987.2011.00984.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T08:18:12Z","timestamp":1697271492000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1744-9987.2011.00984.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6,9]]},"references-count":10,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,10]]}},"alternative-id":["10.1111\/j.1744-9987.2011.00984.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1744-9987.2011.00984.x","archive":["Portico"],"relation":{},"ISSN":["1744-9979","1744-9987"],"issn-type":[{"value":"1744-9979","type":"print"},{"value":"1744-9987","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,6,9]]}}}