{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:20:45Z","timestamp":1771003245285,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,10,5]],"date-time":"2007-10-05T00:00:00Z","timestamp":1191542400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p>The cytotoxic, anti\u2010proliferative and apoptotic effects of 3\u2010Bromoacetoxy Calcidiol (B3CD), a derivative of vitamin D<jats:sub>3<\/jats:sub> precursor calcidiol, on human neuroblastoma (NB) cells were examined. NB, predominantly a tumor of early childhood, is the most common extracranial solid tumor. Despite aggressive treatments, survival for advanced stages remains low and novel treatment strategies are needed. B3CD\u2010induced apoptosis in various neuroblastic cells via caspases\u20103 and \u20109 activation. B3CD upregulated mitochondrial pro\u2010apoptotic Bax and anti\u2010apoptotic Bcl\u20102 expression, caused cytochrome <jats:italic>c<\/jats:italic> release, downregulated N\u2010Myc expression and activated pro\u2010survival marker Akt. Accordingly, B3CD treatment dose dependently reduced the viability of NB cells with IC<jats:sub>50<\/jats:sub> values between 1 and 3\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>m<\/jats:sc>. The cytotoxicity of B3CD was significantly higher than for the calcemic parent\u2010compound vitamin D<jats:sub>3<\/jats:sub> (IC<jats:sub>50<\/jats:sub> between 10 and 30\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>m<\/jats:sc>). Further studies revealed that B3CD treatment inhibits the proliferation of NB cells at low concentrations (IC<jats:sub>50<\/jats:sub> between 30 and 100\u2003n<jats:sc>m<\/jats:sc>). Cell cycle analysis showed a dramatic increase in the apoptotic sub\u2010diploidal population along with a cell cycle block. In summary, the present study shows that B3CD is toxic to NB cells via suppression of cell proliferation and cell viability by caspase activation and regulation of survival signals. These results suggest that B3CD could be developed as a treatment for NB.<\/jats:p>","DOI":"10.1111\/j.1747-0285.2007.00567.x","type":"journal-article","created":{"date-parts":[[2007,10,5]],"date-time":"2007-10-05T09:15:38Z","timestamp":1191575738000},"page":"302-310","source":"Crossref","is-referenced-by-count":19,"title":["Anti\u2010proliferative and Pro\u2010apoptotic Properties of 3\u2010Bromoacetoxy Calcidiol in High\u2010risk Neuroblastoma"],"prefix":"10.1111","volume":"70","author":[{"given":"Thilo S.","family":"Lange","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakesh K.","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyu 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