{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:07:20Z","timestamp":1789913240964,"version":"4.0.1"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,4,27]],"date-time":"2011-04-27T00:00:00Z","timestamp":1303862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:p>\n                    Interstitial cystitis\/painful bladder syndrome is a chronic bladder disorder with epithelial thinning or ulceration, pain, urinary frequency and urgency, for which there is no reliably effective therapy. We previously reported that interstitial cystitis\/painful bladder syndrome bladder epithelial cells make a glycopeptide antiproliferative factor or \u2018APF\u2019 (Neu5Ac\u03b12\u20103Gal\u03b21\u20103GalNAc\u03b1\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010TVPAAVVVA) that induces abnormalities in normal cells similar to those in interstitial cystitis\/painful bladder syndrome cells\n                    <jats:italic>in vitro<\/jats:italic>\n                    , including decreased proliferation, decreased tight junction formation, and increased paracellular permeability. We screened inactive APF derivatives for their ability to block antiproliferative activity of asialylated\u2010APF (\u2018\n                    <jats:italic>as<\/jats:italic>\n                    \u2010APF\u2019) in normal bladder cells and determined the ability of\n                    <jats:italic>as<\/jats:italic>\n                    \u2010APF\u2010blocking derivatives to normalize tight junction protein expression, paracellular permeability, and\/or proliferation of interstitial cystitis\/painful bladder syndrome cells. Only two of these derivatives [Gal\u03b21\u20103GalNAc\u03b1\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010TV\u2010(\n                    <jats:sc>d<\/jats:sc>\n                    \u2010pipecolic acid)\u2010AAVVVA and Gal\u03b21\u20103GalNAc\u03b1\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010TV\u2010(\n                    <jats:sc>d<\/jats:sc>\n                    \u2010proline)\u2010AAVVVA] blocked\n                    <jats:italic>as<\/jats:italic>\n                    \u2010APF antiproliferative activity in normal cells (p\u2003&lt;\u20030.001 for both). Both of these antagonists also 1) significantly increased mRNA expression of ZO\u20101, occludin, and claudins 1, 4, 8, and 12 in interstitial cystitis\/painful bladder syndrome cells by qRT\u2010PCR; 2) normalized interstitial cystitis\/painful bladder syndrome epithelial cell tight junction protein expression and tight junction formation by confocal immunofluorescence microscopy; and 3) decreased paracellular permeability of\n                    <jats:sup>14<\/jats:sup>\n                    C\u2010mannitol and\n                    <jats:sup>3<\/jats:sup>\n                    H\u2010inulin between confluent interstitial cystitis\/painful bladder syndrome epithelial cells on Transwell plates, suggesting that these potent APF antagonists may be useful for the development as interstitial cystitis\/painful bladder syndrome therapies.\n                  <\/jats:p>","DOI":"10.1111\/j.1747-0285.2011.01108.x","type":"journal-article","created":{"date-parts":[[2011,2,26]],"date-time":"2011-02-26T12:45:20Z","timestamp":1298724320000},"page":"421-430","source":"Crossref","is-referenced-by-count":28,"title":["Normalization of Proliferation and Tight Junction Formation in Bladder Epithelial Cells from Patients with Interstitial Cystitis\/Painful Bladder Syndrome by\n                    <scp>d<\/scp>\n                    \u2010Proline and\n                    <scp>d<\/scp>\n                    \u2010Pipecolic Acid Derivatives of Antiproliferative Factor"],"prefix":"10.1111","volume":"77","author":[{"given":"Susan","family":"Keay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Kaczmarek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen\u2010Ou","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristopher","family":"Koch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoltan","family":"Szekely","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Joseph J.","family":"Barchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Michejda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,4,27]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)40201-1"},{"key":"e_1_2_8_3_2","first-page":"15","article-title":"Critical notes: respective immune pathogenesis of interstitial cystitis","volume":"13","author":"Skoluda D.","year":"1974","journal-title":"Urologe A"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(01)01166-9"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00307.2001"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00341.2003"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/cbir.2001.0829"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2007.06795.x"},{"key":"e_1_2_8_9_2","first-page":"76","article-title":"Chronic ulcerating interstitial cystitis (Hunner\u2019s ulcer)","volume":"93","author":"Smith B.H.","year":"1972","journal-title":"Arch Pathol"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)38437-9"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000118938.09119.a5"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1983.tb03354.x"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)63178-1"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000180417.11976.99"},{"key":"e_1_2_8_15_2","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S0022-5347(18)30562-7","article-title":"Decreased expression of claudins 1, 4, and 8 in bladder epithelial cell explants from interstitial cystitis as compared to normal controls","volume":"177","author":"Zhang C.\u2010O.","year":"2007","journal-title":"J Urol"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)66980-7"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(01)01127-X"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404509101"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(03)00005-0"},{"key":"e_1_2_8_20_2","first-page":"991","article-title":"Molecular cloning and characterization of human Frizzled\u20108 gene on chromosome 10p11.2","volume":"18","author":"Saitoh T.","year":"2001","journal-title":"Int J Oncol"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.06.058"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00055.2003"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)64198-3"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm8002763"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)41529-1"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.21266"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.430350105"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867024606731"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar8000052"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm010096n"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604581200"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-6-186"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90264.2008"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(02)02280-X"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2613.2002.00208.x"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20687"},{"key":"e_1_2_8_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-007-0205-4"},{"key":"e_1_2_8_38_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00084.2009"},{"key":"e_1_2_8_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2008.08097.x"},{"key":"e_1_2_8_40_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E08-08-0849"}],"container-title":["Chemical Biology &amp; Drug Design"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1747-0285.2011.01108.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1747-0285.2011.01108.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T00:47:55Z","timestamp":1697330875000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1747-0285.2011.01108.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4,27]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,6]]}},"alternative-id":["10.1111\/j.1747-0285.2011.01108.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1747-0285.2011.01108.x","archive":["Portico"],"relation":{},"ISSN":["1747-0277","1747-0285"],"issn-type":[{"value":"1747-0277","type":"print"},{"value":"1747-0285","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,4,27]]}}}