{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T05:59:26Z","timestamp":1775195966577,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2006,5,22]],"date-time":"2006-05-22T00:00:00Z","timestamp":1148256000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim:\u2002<\/jats:bold> This study was performed to examine thyrotropin\u2010releasing hormone (TRH) secretion and regulation in rat heart.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Expression of prepro\u2010TRH gene in left atrium and left ventricle was studied by RT\u2010PCR. TRH secretion from slices of left auricle and left ventricle in response to cell swelling (induced by hypotonic medium or ethanol in isosmotic medium), angiotensin II and losartan and their combinations was studied.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> RT\u2010PCR revealed two times higher prepro\u2010TRH expression in left auricle than left ventricle. In transgenic rats with extra copy of mouse renin gene a marked increase of prepro\u2010TRH expression in the heart was noted but the relative difference between left atrium and left ventricle persisted. The swelling stimulated TRH release from both left auricle and left ventricle and this stimulation could not be inhibited by bumetanide. Angiotensin II (10\u2003nmol\u2003L<jats:sup>\u22121<\/jats:sup>) added into medium significantly decreased basal secretion of TRH. The inhibiting effect of Angiotensin II was prevented by 1\u2003<jats:italic>\u03bc<\/jats:italic>mol\u2003L<jats:sup>\u22121<\/jats:sup> losartan, an angiotensin II AT1 receptor blocker. When angiotensin II and hypotonicity were applied simultaneously, swelling\u2010induced secretion persisted.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> TRH secretion from heart slices has attributes of regulated secretion \u2013 depending on the stimulus it could be either stimulated or inhibited. Renin positively affects prepro\u2010TRH expression in the heart. Angiotensin II inhibits TRH secretion from heart tissue by a mechanism involving AT1 receptors. Swelling\u2010induced TRH secretion overrides inhibitory effect of angiotensin II. Swelling could be a useful tool when natural or pharmacological secretagogue is unknown. Peptides and proteins released by swelling could be mediators of local and remote ischaemic preconditioning protecting from subsequent ischaemia.<\/jats:p>","DOI":"10.1111\/j.1748-1716.2006.01545.x","type":"journal-article","created":{"date-parts":[[2006,5,22]],"date-time":"2006-05-22T10:12:03Z","timestamp":1148292723000},"page":"313-319","source":"Crossref","is-referenced-by-count":10,"title":["Thyrotropin\u2010releasing hormone in rat heart: effect of swelling, angiotensin II and renin gene"],"prefix":"10.1111","volume":"187","author":[{"given":"Z.","family":"Ba\u010dova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Baqi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Be\u0148a\u010dka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Payer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Kri\u017eanov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Zeman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Smrekov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0160.","family":"Zorad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"\u0160trb\u00e1k","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02314-9"},{"key":"e_1_2_7_3_1","first-page":"181","article-title":"Effect of neonatal streptozotocin (STZ) and thyrotropin\u2010releasing hormone (TRH) treatments on insulin secretion in adult rats","volume":"24","author":"Bacova Z.","year":"2005","journal-title":"Gen Physiol Biophys"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000087732"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(87)90094-5"},{"key":"e_1_2_7_6_1","first-page":"567","article-title":"Proteins released from liver after ischaemia induced an elevation of heart resistance against ischaemia\u2010reperfusion injury: 1. Beneficial effect of protein fraction isolated from perfusate after ischaemia and reperfusion of liver","volume":"22","author":"Bartekova M.","year":"2003","journal-title":"Gen Physiol Biophys"},{"key":"e_1_2_7_7_1","first-page":"489","article-title":"Proteins released from liver after ischaemia induced an elevation of heart resistance against ischaemia\u2010reperfusion injury: 2. Beneficial effect of liver ischaemia in situ","volume":"23","author":"Bartekova M.","year":"2004","journal-title":"Gen Physiol Biophys"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1420060"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.25.5.1014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4179(92)90047-Z"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70325-7"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-123-6-2805"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.103.5.736"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000127951.13380.B4"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-005-4008-0"},{"key":"e_1_2_7_16_1","first-page":"237","article-title":"Effect of two distinct stressors on gene expression of the type 1 IP3 receptors","volume":"24","author":"Krizanova O.","year":"2005","journal-title":"Gen Physiol Biophys"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000070255"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000164066"},{"key":"e_1_2_7_19_1","first-page":"309","article-title":"Positive inotropic effect of thyrotropin\u2010releasing hormone on isolated rat hearts","volume":"15","author":"Socci R.","year":"1996","journal-title":"Gen Physiol Biophys"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000016372"},{"key":"e_1_2_7_21_1","first-page":"325","volume-title":"Cell Volume and Signaling, Advances in Experimental Medicine and Biology","author":"Strbak V.","year":"2004"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.24.4.445"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.1998.8.897"},{"key":"e_1_2_7_24_1","first-page":"224","article-title":"Targeting ischaemia \u2013 cell swelling and drug efficacy","volume":"18","author":"Wright A.R.","year":"1997","journal-title":"Trends Pharmacol Sci"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1748-1716.2006.01545.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1748-1716.2006.01545.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T19:42:34Z","timestamp":1693424554000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1748-1716.2006.01545.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":23,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1748-1716.2006.01545.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1748-1716.2006.01545.x","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5]]}}}