{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:01:57Z","timestamp":1762160517012},"reference-count":79,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2006,5,22]],"date-time":"2006-05-22T00:00:00Z","timestamp":1148256000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aqueous humour is secreted by the ciliary epithelium comprising pigmented and non\u2010pigmented cell layers facing the stroma and aqueous humour respectively. Net chloride secretion likely limits the rate of aqueous humour formation and proceeds in three steps: stromal chloride entry into pigmented cells, diffusion through gap junctions and final non\u2010pigmented cell secretion. Swelling\u2010activated chloride channels function on both epithelial surfaces. At the stromal surface, swelling\u2010 and cyclic adenosine monophosphate\u2010activated maxi\u2010chloride channels can recycle chloride, reducing net chloride secretion. At the aqueous\u2010humour surface, swelling\u2010 and A<jats:sub>3<\/jats:sub> adenosine receptor\u2010activated chloride channels subserve chloride release into the aqueous humour. The similar macroscopic properties of the two non\u2010pigmented cell chloride currents suggest that both flow through a common conduit. In addition, measurements of intraocular pressure (IOP) in living wild\u2010type and mutant mice have confirmed that A<jats:sub>3<\/jats:sub> adenosine receptor\u2010activated agonists and antagonists increase and lower IOP respectively. Isolated ciliary epithelial cells are commonly perfused with hypotonic solution to probe and characterize chloride channels, but the physiological role of swelling\u2010activated channels has been unclear without knowing their epithelial distribution. Recently, hypotonic challenge has been found to stimulate the chloride\u2010sensitive short\u2010circuit current across the intact bovine ciliary epithelium, suggesting that the net effect of the swelling\u2010activated chloride currents is oriented to enhance aqueous humour formation. Taken together, the results suggest that swelling\u2010activated chloride channels are predominantly oriented to enhance aqueous humour secretion, and these chloride channels at the aqueous surface may be identical with adenosine receptor\u2010activated chloride channels which likely modulate aqueous inflow and IOP in the living mouse.<\/jats:p>","DOI":"10.1111\/j.1748-1716.2006.01548.x","type":"journal-article","created":{"date-parts":[[2006,5,22]],"date-time":"2006-05-22T10:12:03Z","timestamp":1148292723000},"page":"345-352","source":"Crossref","is-referenced-by-count":18,"title":["Swelling\u2010activated chloride channels in aqueous humour formation: on the one side and the other"],"prefix":"10.1111","volume":"187","author":[{"given":"C. W.","family":"Do","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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