{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:32:51Z","timestamp":1787067171896,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2006,10,20]],"date-time":"2006-10-20T00:00:00Z","timestamp":1161302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Aim:\u2002<\/jats:bold>\n                    To determine whether reduced cardiomyocyte contractility in heart failure is associated with reduced intracellular pH (pH\n                    <jats:sub>i<\/jats:sub>\n                    ). Involvement of the Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchanger and the H\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    ATPase were investigated with specific blockers.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:\u2002<\/jats:bold>\n                    Myocardial infarction and subsequent heart failure in Sprague\u2013Dawley rats were induced by chronic occlusion of the left coronary artery. 6\u2003weeks post\u2010ligation, contractility (cell shortening) and pH\n                    <jats:sub>i<\/jats:sub>\n                    (BCECF fluorescence) were recorded in freshly dissociated cardiomyocytes during 2\u201310\u2003Hz electrical stimulation, with or without either Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchanger or H\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    ATPase inhibition.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:\u2002<\/jats:bold>\n                    Elevated end\u2010diastolic and reduced peak systolic pressures confirmed heart failure. Increased heart weights (20\u201330%;\n                    <jats:italic>P<\/jats:italic>\n                    \u2003\u2264\u20030.01) and cardiomyocyte lengths and widths (22\u201325%;\n                    <jats:italic>P<\/jats:italic>\n                    \u2003\u2264\u20030.01) confirmed substantial cardiac hypertrophy. In myocytes isolated from sham operated rats, a positive staircase response occurred with stimulation rates from 2 to 7\u2003Hz; further increases in stimulation rate up to 10\u2003Hz reduced contractility. In contrast, pH\n                    <jats:sub>i<\/jats:sub>\n                    fell progressively over the entire stimulation range. In failing myocytes, pH\n                    <jats:sub>i<\/jats:sub>\n                    was consistently 0.07 pH units lower and contractility 40% lower (\n                    <jats:italic>P<\/jats:italic>\n                    \u2003\u2264\u20030.01) than sham control values; the shape of the contractility staircase remained similar to controls. At all stimulation frequencies, Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchanger inhibition reduced pH\n                    <jats:sub>i<\/jats:sub>\n                    by 0.05 pH units (\n                    <jats:italic>P<\/jats:italic>\n                    \u2003\u2264\u20030.01) and contractility by 22% (\n                    <jats:italic>P<\/jats:italic>\n                    \u2003\u2264\u20030.05) in cardiomyocytes from the heart failure group. A significantly smaller decrease of pH\n                    <jats:sub>i<\/jats:sub>\n                    and reduction in contractility was observed after inhibition of Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchanger (10\u2003\n                    <jats:italic>\u03bc<\/jats:italic>\n                    <jats:sc>m<\/jats:sc>\n                    HOE694) in sham myocytes. H\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    ATPase inhibition (100\u2003\n                    <jats:italic>\u03bc<\/jats:italic>\n                    <jats:sc>m<\/jats:sc>\n                    SCH28080) had no effect on pH\n                    <jats:sub>i<\/jats:sub>\n                    .\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:\u2002<\/jats:bold>\n                    Reduced pH\n                    <jats:sub>i<\/jats:sub>\n                    is accompanied by reduced cardiomyocyte contractility in isolated myocytes from post\u2010MI heart failure. The data suggest compensatory Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchanger activation in heart failure, whereas H\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    ATPase does not appear to contribute significantly to pH\n                    <jats:sub>i<\/jats:sub>\n                    maintenance.\n                  <\/jats:p>","DOI":"10.1111\/j.1748-1716.2006.01621.x","type":"journal-article","created":{"date-parts":[[2006,10,20]],"date-time":"2006-10-20T06:55:39Z","timestamp":1161327339000},"page":"185-193","source":"Crossref","is-referenced-by-count":11,"title":["Reduced pH and contractility in failing rat cardiomyocytes"],"prefix":"10.1111","volume":"188","author":[{"given":"O. J.","family":"Kemi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Arbo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"H\u00f8ydal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. P.","family":"Loennechen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Wisl\u00f8ff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. L.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d8.","family":"Ellingsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0001-6772.2003.01191.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.55.3.382"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"H131","DOI":"10.1152\/ajpheart.2002.282.1.H131","article-title":"Beneficial effects of the Ca2+ sensitizer levosimendan in human myocardium","volume":"282","author":"Brixius K.","year":"2002","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.79.4.698"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00602.2003"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000016701.85760.97"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00795409"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1978.sp012231"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240000512"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.82.4.1266"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114611"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.14.1679"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.98.20.2141"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.275.3.H977"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc3201.092286"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.85.9.777"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0159z.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00075198-200310000-00002"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.89.1.345"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.6.H2902"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc1102.105258"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.02.031"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1536\/jhj.40.351"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.258.6.C967"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.99.8.1077"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(96)79711-4"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000093277.20968.C7"},{"key":"e_1_2_8_29_1","first-page":"1","volume":"555","author":"Ventura\u2010Clapier R.","year":"2004","journal-title":"Energy metabolism in heart failure"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1997.sp004011"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(01)00210-3"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(01)00565-X"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2005.06.040"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00277.2003"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1999.86.3.943"}],"container-title":["Acta Physiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1748-1716.2006.01621.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1748-1716.2006.01621.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T12:11:43Z","timestamp":1693483903000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1748-1716.2006.01621.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,20]]},"references-count":34,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1111\/j.1748-1716.2006.01621.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1748-1716.2006.01621.x","archive":["Portico"],"relation":{},"ISSN":["1748-1708","1748-1716"],"issn-type":[{"value":"1748-1708","type":"print"},{"value":"1748-1716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10,20]]}}}