{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T20:10:29Z","timestamp":1764101429603,"version":"3.40.3"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T00:00:00Z","timestamp":1337040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this work was to study antagonistic interactions between the effects of various types of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channel blockers and isoproterenol on the amplitude of<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\u2010type<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>current in canine ventricular cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Whole\u2010cell version of the patch clamp technique was used to study the effect of isoproterenol on<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>current in the absence and presence of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channel\u2013blocking agents, including nifedipine, nisoldipine, diltiazem, verapamil,<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>o<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>and<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five micromolar<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>ifedipine, 1\u00a0\u03bc<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>nisoldipine, 10\u00a0\u03bc<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>diltiazem, 5\u00a0\u03bc<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>verapamil, 3\u00a0m<jats:styled-content style=\"fixed-case\">M C<\/jats:styled-content>o<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>and 5\u00a0m<jats:styled-content style=\"fixed-case\">M M<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>evoked uniformly a 90\u201395% blockade of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>current in the absence of isoproterenol. Isoproterenol (100\u00a0n<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>) alone increased the amplitude of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>current from 6.8\u00a0\u00b1\u00a01.3 to 23.7\u00a0\u00b1\u00a02.2<jats:styled-content style=\"fixed-case\">pA<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">pF<\/jats:styled-content>in a reversible manner. Isoproterenol caused a marked enhancement of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>current even in the presence of nifedipine, nisoldipine, diltiazem and verapamil, but not in the presence of<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>o<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>or<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results indicate that the action of isoproterenol is different in the presence of organic and inorganic<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channel blockers.<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>o<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>and<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>l<jats:sub>2<\/jats:sub>were able to fully prevent the effect of isoproterenol on<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>current, while the organic<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channel blockers failed to do so. This has to be born in mind when the effects of organic<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup>channel blockers are evaluated either experimentally or clinically under conditions of increased sympathetic tone.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1748-1716.2012.02448.x","type":"journal-article","created":{"date-parts":[[2012,4,23]],"date-time":"2012-04-23T07:43:31Z","timestamp":1335167011000},"page":"42-50","source":"Crossref","is-referenced-by-count":4,"title":["Interaction between<scp>C<\/scp>a<sup>2+<\/sup>channel blockers and isoproterenol on<scp>L<\/scp>\u2010type<scp>C<\/scp>a<sup>2+<\/sup>current in canine ventricular cardiomyocytes"],"prefix":"10.1111","volume":"206","author":[{"given":"V.","family":"Farkas","sequence":"first","affiliation":[{"name":"Department of Dentistry University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Szentandr\u00e1ssy","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"B\u00e1r\u00e1ndi","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Hegyi","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Ruzsnavszky","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Ruzsnavszky","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Horv\u00e1th","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"B\u00e1ny\u00e1sz","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Magyar","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"M\u00e1rton","sequence":"additional","affiliation":[{"name":"Department of Dentistry University of Debrecen Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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