{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T13:11:44Z","timestamp":1792501904377,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,5,4]],"date-time":"2012-05-04T00:00:00Z","timestamp":1336089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:p><jats:italic>Please cite this paper as:<\/jats:italic> Van Poucke <jats:italic>et\u00a0al.<\/jats:italic> (2013) Effect of receptor specificity of A\/Hong Kong\/1\/68 (H3N2) influenza virus variants on replication and transmission in pigs. Influenza and Other Respiratory Viruses 7(2) 151\u2013159.<\/jats:p><jats:p><jats:bold>Background\u2002<\/jats:bold> Several arguments plead for an important role of pigs in human influenza ecology, including the similar receptor expression pattern in the respiratory tract of both species. How virus receptor binding specificity affects transmission in pigs, on the other hand, has not been studied so far.<\/jats:p><jats:p><jats:bold>Objectives\u2002<\/jats:bold> Using recombinant viruses R1\u2010HK, which harbored all genes from the original pandemic virus A\/Hong Kong\/1\/68 (H3N2), and R2\u2010HK, which differed by L226Q and S228G mutations in the hemagglutinin and conversion to an avian\u2010virus\u2010like receptor specificity, we assessed the role of receptor specificity on (i) replication in porcine respiratory explants, (ii) pig\u2010to\u2010pig transmission, and (iii) replication and organ tropism in pigs.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> In nasal, tracheal, and bronchial explants, we noticed a 10\u2010 to 100\u2010fold lower replication of R2\u2010HK compared with R1\u2010HK. In the lung explants, the viruses replicated with comparable efficiency. These observations correlated with the known expression level of Sia\u03b12,3\u2010galactose in these tissues. In the pathogenesis study, virus titers in the respiratory part of the nasal mucosa, the trachea, and the bronchus were in line with the <jats:italic>ex vivo<\/jats:italic> results. R2\u2010HK replicated less efficiently in the lungs of pigs than R1\u2010HK, which contrasted with the explants results. R2\u2010HK also showed a pronounced tropism for the olfactory part of the nasal mucosa. Transmissibility experiments revealed that pig\u2010to\u2010pig transmission was abrogated when the virus obtained Sia\u03b12,3\u2010galactose binding preference.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Our data suggest that Sia\u03b12,6\u2010galactose binding is required for efficient transmission in pigs.<\/jats:p>","DOI":"10.1111\/j.1750-2659.2012.00376.x","type":"journal-article","created":{"date-parts":[[2012,5,4]],"date-time":"2012-05-04T12:57:51Z","timestamp":1336136271000},"page":"151-159","source":"Crossref","is-referenced-by-count":14,"title":["Effect of receptor specificity of A\/Hong Kong\/1\/68 (H3N2) influenza virus variants on replication and transmission in pigs"],"prefix":"10.1111","volume":"7","author":[{"given":"Sjouke","family":"Van 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