{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:24:32Z","timestamp":1791285872923,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T00:00:00Z","timestamp":1157500800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p><jats:bold>Objective\u2003\u2003<\/jats:bold> To detect <jats:italic>Anaplasma platys<\/jats:italic> and <jats:italic>Babesia canis vogeli<\/jats:italic> infection, using polymerase chain reaction (PCR)\u2010based assays, in free\u2010roaming dogs associated with eight Aboriginal communities in remote areas of Australia and to determine the impact of infection through the assessment of platelet numbers.<\/jats:p><jats:p><jats:bold>Procedures\u2003\u2003<\/jats:bold> Blood samples from 215 dogs were screened by PCR for <jats:italic>A platys<\/jats:italic> and <jats:italic>B canis vogeli<\/jats:italic> using established genus\u2010specific DNA primers for the 16S and 18S rRNA genes respectively. Both <jats:italic>A platys<\/jats:italic> DNA and <jats:italic>B canis vogeli<\/jats:italic> DNA were confirmed from the screening PCR either by sequencing or by the use of species\u2010specific primers. Peripheral blood films from 92 of the 215 dogs were used to estimate platelet numbers through an indirect method.<\/jats:p><jats:p><jats:bold>Results\u2003\u2003<\/jats:bold> Of 215 dogs, 69 (32%) were positive for <jats:italic>A platys<\/jats:italic>, 22 (10%) for <jats:italic>B canis vogeli<\/jats:italic> and 24 (11%) for both. The two organisms were detected singularly and as coinfection in all communities. For the 92 dogs in which peripheral blood films were examined, the mean estimated platelet counts for the non\u2010infected dogs was 318 \u00d7 10<jats:sup>9<\/jats:sup>\/L, those infected with <jats:italic>A platys<\/jats:italic> alone was 256 \u00d7 10<jats:sup>9<\/jats:sup>\/L, those with <jats:italic>B canis vogeli<\/jats:italic> alone was 276 \u00d7 10<jats:sup>9<\/jats:sup>\/L and those infected with both parasites was 169 \u00d7 10<jats:sup>9<\/jats:sup>\/L. In young dogs, infection produced significantly decreased mean platelet counts when compared to uninfected dogs. Thrombocytopenia (&lt;\u00a0200 \u00d7 10<jats:sup>9<\/jats:sup>\/L) was detected in 18 (51%) dogs infected with <jats:italic>A platys<\/jats:italic> alone, 3 (33%) dogs infected with <jats:italic>B canis vogeli<\/jats:italic> alone, 13 (72%) dogs coinfected, and 8 (27%) uninfected dogs.<\/jats:p><jats:p><jats:bold>Conclusions\u2003\u2003<\/jats:bold>  <jats:italic>A platys<\/jats:italic> and <jats:italic>B canis vogeli<\/jats:italic> infection, either singularly or together, was widespread in free roaming dogs associated with remote Aboriginal communities in the Northern Territory and north\u2010western New South Wales. Moreover, both <jats:italic>A platys<\/jats:italic> and <jats:italic>B canis vogeli<\/jats:italic> infections were associated with a reduction in mean platelet numbers in dog populations, particularly in young dogs. The fact that 51% of dogs infected with <jats:italic>A platys<\/jats:italic> alone and 72% dogs coinfected were thrombocytopenic compared to 27% of uninfected dogs suggests that the organism alone or in combination with <jats:italic>B canis vogeli<\/jats:italic> has the potential to cause thrombocytopenia and perhaps contribute to a clinical bleeding disorder in infected dogs.<\/jats:p>","DOI":"10.1111\/j.1751-0813.2006.00029.x","type":"journal-article","created":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T13:06:48Z","timestamp":1157548008000},"page":"321-325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Detection of <i>Anaplasma platys<\/i> and <i>Babesia canis vogeli<\/i> and their impact on platelet numbers in free\u2010roaming dogs associated with remote Aboriginal communities in Australia"],"prefix":"10.1111","volume":"84","author":[{"given":"GK","family":"BROWN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PJ","family":"CANFIELD","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RH","family":"DUNSTAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TK","family":"ROBERTS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AR","family":"MARTIN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CS","family":"BROWN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"IRVING","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,9,6]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/137.2.182"},{"key":"e_1_2_7_3_2","first-page":"106","article-title":"Rickettsial infections in Dogs Part II. 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