{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T17:19:27Z","timestamp":1787678367268,"version":"build-2784847793"},"reference-count":24,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T00:00:00Z","timestamp":1171411200000},"content-version":"vor","delay-in-days":44,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:p><jats:bold>Objective\u2003<\/jats:bold>To develop a multiplex polymerase chain reaction (PCR) assay for the rapid detection of<jats:italic>Brucella ovis<\/jats:italic>,<jats:italic>Actinobacillus seminis<\/jats:italic>,<jats:italic>Histophilus somni<\/jats:italic>in fresh ram semen samples.<\/jats:p><jats:p><jats:bold>Design\u2003<\/jats:bold>The multiplex assay was based on the single PCR assays published for the detection of<jats:italic>A seminis<\/jats:italic>and<jats:italic>B ovis<\/jats:italic>, and the forward primer published for the detection of<jats:italic>H somni<\/jats:italic>; an alternative reverse primer for<jats:italic>H somni<\/jats:italic>was designed in this study.<\/jats:p><jats:p><jats:bold>Procedure\u2003<\/jats:bold>Culture and PCR of 295 fresh semen samples were carried out.<\/jats:p><jats:p><jats:bold>Results\u2003<\/jats:bold>The multiplex PCR was far more successful in the detection of<jats:italic>H somni<\/jats:italic>(45\/295) than culture (23\/295).<jats:italic>A seminis<\/jats:italic>was also detected in more semen samples by multiplex PCR (29\/295) than culture (13\/295) and<jats:italic>B ovis<\/jats:italic>was detected in three samples using both PCR and culture. No amplifications were detected with DNA from a range of bacterial isolates including species associated with epididymitis in rams.<\/jats:p><jats:p><jats:bold>Conclusion\u2003<\/jats:bold>This PCR could be used as a complementary test, or alternative to culture of ram semen and other biological samples for the detection<jats:italic>B ovis<\/jats:italic>,<jats:italic>H somni<\/jats:italic>and<jats:italic>A seminis<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1751-0813.2006.00098.x","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T01:33:17Z","timestamp":1171503197000},"page":"72-77","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Multiplex PCR for the detection of<i>Brucella ovis<\/i>,<i>Actinobacillus seminis<\/i>and<i>Histophilus somni<\/i>in ram semen"],"prefix":"10.1111","volume":"85","author":[{"given":"VF","family":"Saunders","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LA","family":"Reddacliff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Hornitzky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,2,14]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(82)90049-9"},{"key":"e_1_2_7_3_2","first-page":"393","article-title":"Association of age of ram with distribution of epididymal lesions and etiologic agent","volume":"188","author":"Walker RL","year":"1986","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1978.tb00291.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1987.tb09640.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.135.11.254"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1982.tb00568.x"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.125.20.504"},{"key":"e_1_2_7_9_2","doi-asserted-by":"crossref","first-page":"313","DOI":"10.2460\/javma.1990.196.02.313","article-title":"Brucella ovis excretion in semen of seronegative, clinically normal breeding rams","volume":"196","author":"Bulgin MS","year":"1990","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.129.14.304"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02637-0"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1991.tb03104.x"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1977.tb00134.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1978.tb05567.x"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1990.tb01095.x"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(98)00222-3"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1998.tb05257.x"},{"key":"e_1_2_7_18_2","first-page":"155","article-title":"Comparative study of culture media for isolating Brucella ovis","volume":"23","author":"Paolicchi F","year":"1991","journal-title":"Rev Argent Microbiol"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00310-3"},{"key":"e_1_2_7_20_2","first-page":"137","volume-title":"Bergey's Manual of Determinative Bacteriology","author":"Holt JG","year":"1994"},{"key":"e_1_2_7_21_2","volume-title":"Current protocols in molecular biology","author":"Ausabel FM","year":"1994"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(98)00252-1"},{"key":"e_1_2_7_23_2","first-page":"173","article-title":"A comparative study of bovine and ovine Haemophilus somnus isolates","volume":"59","author":"Ward AC","year":"1995","journal-title":"Can J Vet Res"},{"key":"e_1_2_7_24_2","first-page":"140","article-title":"The use of gene amplification for the detection of the Aujeszky's disease virus in the sperm of boars","volume":"26","author":"Guerin C","year":"1995","journal-title":"Vet Res"},{"key":"e_1_2_7_25_2","first-page":"1928","article-title":"Prevalence of Histophilus ovis and Actinobacillus seminis in the genital tract of sheep","volume":"47","author":"Walker RL","year":"1986","journal-title":"Am J Vet Res"}],"updated-by":[{"DOI":"10.1111\/j.1751-0813.2007.00157.x","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2008,10,9]],"date-time":"2008-10-09T00:00:00Z","timestamp":1223510400000}}],"container-title":["Australian Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1751-0813.2006.00098.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1751-0813.2006.00098.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T23:39:28Z","timestamp":1707608368000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1751-0813.2006.00098.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":24,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1111\/j.1751-0813.2006.00098.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1751-0813.2006.00098.x","archive":["Portico"],"relation":{},"ISSN":["0005-0423","1751-0813"],"issn-type":[{"value":"0005-0423","type":"print"},{"value":"1751-0813","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1]]},"assertion":[{"value":"2007-02-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}