{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T15:59:58Z","timestamp":1791734398791,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,9,18]],"date-time":"2012-09-18T00:00:00Z","timestamp":1347926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photochem &amp; Photobiology"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A major difficulty in photodynamic therapy is the poor solubility of the photosensitizer (<jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content>) under physiological conditions which correlates with low bioavailability. <jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content> aggregation leads to a decrease in the photodynamic efficiency and a more limited activity <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. To improve the aqueous solubility and reduce the aggregation of 2,9(10),16(17),23(24)\u2010tetrakis[(2\u2010dimethylamino)ethylsulfanyl]phthal\u2010ocyaninatozinc(<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>) (Pc9), the encapsulation into four poloxamine polymeric micelles (T304, T904, T1107 and T1307) displaying a broad spectrum of molecular weight and hydrophilic\u2013lipophilic balance was investigated. The aqueous solubility of Pc9 was increased up to 30 times. Morphological evaluation showed the formation of Pc9\u2010loaded spherical micelles in the nanosize range. <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">Vis<\/jats:styled-content> and fluorescence studies indicated that Pc9 is less aggregated upon encapsulation in comparison with Pc9 in water\u2013<jats:styled-content style=\"fixed-case\">DMSO<\/jats:styled-content> 2% and remained photostable. Pc9\u2010loaded micelles generated singlet molecular oxygen in high yields. Photocytotoxicity assays using human nasopharynx <jats:styled-content style=\"fixed-case\">KB<\/jats:styled-content> carcinoma cells confirmed that the encapsulation of Pc9 in T1107 and T1307 increases its photocytotoxicity by 10 times in comparison with the free form in water\u2013<jats:styled-content style=\"fixed-case\">DMSO<\/jats:styled-content>. In addition, Pc9 incorporated into cells was mainly localized in lysosomes.<\/jats:p>","DOI":"10.1111\/j.1751-1097.2012.01229.x","type":"journal-article","created":{"date-parts":[[2012,8,27]],"date-time":"2012-08-27T21:07:08Z","timestamp":1346101628000},"page":"492-500","source":"Crossref","is-referenced-by-count":31,"title":["Photodynamic Effects of Zinc(<scp>II<\/scp>) Phthalocyanine\u2010Loaded Polymeric Micelles in Human Nasopharynx <scp>KB<\/scp> Carcinoma Cells"],"prefix":"10.1111","volume":"89","author":[{"given":"Mar\u00eda C.","family":"Garc\u00eda Vior","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e1nica Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julieta","family":"Marino","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas (UBA\u2010CONICET) Facultad de Farmacia y Bioqu\u00edmica Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonor P.","family":"Roguin","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas (UBA\u2010CONICET) Facultad de Farmacia y Bioqu\u00edmica Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Sosnik","sequence":"additional","affiliation":[{"name":"BIONIMED Departamento de Tecnolog\u00eda Farmac\u00e9utica Facultad de Farmacia y Bioqu\u00edmica Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefina","family":"Awruch","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e1nica Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/90.12.889"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm040074b"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(97)00128-0"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9101(2000)26:3<308::AID-LSM9>3.0.CO;2-B"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(96)07370-8"},{"key":"e_1_2_5_7_1","first-page":"185","article-title":"Photodynamic therapy: Selective uptake of photosensitizing drugs into tumor cells","volume":"8","author":"Stockert J. 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