{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T22:00:40Z","timestamp":1778709640476,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,3,27]],"date-time":"2012-03-27T00:00:00Z","timestamp":1332806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Japanese encephalitis is a major public health problem in South\u2010East Asia and Western Pacific countries. The recombinant nonstructural 1 (rNS1) protein of Japanese encephalitis virus is a potential diagnostic as well as vaccine candidate. Developments of cost\u2010effective and simple culture media as well as appropriate culture conditions are generally favourable for large\u2010scale production of recombinant proteins. The effects of medium composition and cultivation conditions on the production of rNS1 protein were investigated in shake flask culture as well as batch cultivation of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . Further, the fed\u2010batch process was also carried out for high cell density cultivation (HCDC) of\n                    <jats:italic>E.\u2003coli<\/jats:italic>\n                    expressing rNS1 protein. Isopropyl\u2010\u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010thiogalactopyranoside (IPTG) was used to induce the expression of rNS1 protein at \u223c\u200313\u2003g dry cell weight per litre of culture. The final dry cell weight after fed\u2010batch cultivation was \u223c\u200317\u2003g\u2003l\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The Inclusion bodies were isolated and purified through affinity chromatography to give a final product yield of \u223c\u2003142\u2003mg\u2003l\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The reactivity of purified protein was confirmed by Western blotting and Enzyme linked immunosorbent assay. These results show that rNS1 protein may be used as a diagnostic reagent or for further prophylactic studies. This approach of producing rNS1 protein in\n                    <jats:italic>E.\u2003coli<\/jats:italic>\n                    with high yield may also offer promising method for production of other viral recombinant proteins.\n                  <\/jats:p>","DOI":"10.1111\/j.1751-7915.2012.00344.x","type":"journal-article","created":{"date-parts":[[2012,3,27]],"date-time":"2012-03-27T16:50:42Z","timestamp":1332867042000},"page":"599-606","source":"Crossref","is-referenced-by-count":10,"title":["Production of recombinant nonstructural 1 protein in\n                    <i>Escherichia coli<\/i>\n                    for early detection of Japanese encephalitis virus infection"],"prefix":"10.1111","volume":"5","author":[{"given":"Nagesh K.","family":"Tripathi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyoti S.","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karttik C.","family":"Biswal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. V. Lakshmana","family":"Rao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.04.003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.07.002"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-010-0753-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2009.02.003"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-7-249"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.2072"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-008-0408-8"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.09.064"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5087-5093.2004"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.22097"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002530050009"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-007-9529-9"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1128\/JVI.72.1.191-200.1998","article-title":"DNA immunization with Japanese encephalitis virus nonstructural protein NS1 elicits protective immunity in mice","volume":"72","author":"Lin Y.L.","year":"1998","journal-title":"J Virol"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-005-0046-3"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(97)00020-0"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2005.11.004"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.4103\/0022-3859.40959"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1263\/jbb.99.303"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-59403-8_9"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.14429\/dsj.59.1501"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2488-4"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201000399"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.09.015"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1751-7915.2012.00344.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1751-7915.2012.00344.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1751-7915.2012.00344.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:24:52Z","timestamp":1762280692000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/j.1751-7915.2012.00344.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,27]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1111\/j.1751-7915.2012.00344.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1751-7915.2012.00344.x","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,27]]}}}