{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T04:15:29Z","timestamp":1744172129898,"version":"3.40.3"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T00:00:00Z","timestamp":1347408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p><jats:bold>Purpose:\u2002<\/jats:bold><jats:italic>N<\/jats:italic>\u2010chlorotaurine (NCT) and its analogues<jats:italic>N<\/jats:italic>\u2010monochloro\u20102,2\u2010dimethyltaurine (NVC\u2010612) and<jats:italic>N<\/jats:italic>\u2010dichloro\u20102,2\u2010dimethyltaurine (NVC\u2010422) are new anti\u2010infectives for topical treatment for conjunctivitis. The aim of this study was to show that these compounds are safe in an EpiOcular model and effective in corneas infected<jats:italic>ex vivo<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>Corneal buttons were excised from porcine eyes. In 183 of the 229 corneas, erosion and artificial superficial stromal incision were induced. They were bathed in suspensions of<jats:italic>Pseudomonas aeruginosa<\/jats:italic>or<jats:italic>Staphylococcus aureus<\/jats:italic>for 24\u2003hr at 37\u00b0C and incubated in solutions of the test substances at 37\u00b0C and pH 7.1. Subsequently, they were subjected to histology (<jats:italic>n<\/jats:italic>\u2003=\u200320) or homogenized followed by quantitative bacterial cultures (<jats:italic>n<\/jats:italic>\u2003=\u2003209). Ocular irritation was tested using the EpiOcular\u2122 tissue system (MatTek Corporation).<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>Bacterial accumulations were detected histologically both on the corneal surface and also in the anterior third of the stroma of incised corneal buttons. All three test compounds at a concentration of 55\u2003m<jats:sc>m<\/jats:sc>(equals 1% NCT) reduced the bacterial counts of<jats:italic>P.\u00a0aeruginosa<\/jats:italic>and<jats:italic>S.\u00a0aureus<\/jats:italic>by approximately 5 log10 after 60\u2010 and 120\u2010min incubation, respectively. Significant killing was observed as early as after 5\u2010min incubation. Also intrastromal bacteria were inactivated. In the EpiOcular\u2122 tissue model, NCT, NVC\u2010422 and NVC\u2010612 had no or very low potential to irritate corneal tissue.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold><jats:italic>N<\/jats:italic>\u2010chlorotaurine, NVC\u2010422 and NVC\u2010612 are non\u2010irritating in cornea and kill<jats:italic>P.\u00a0aeruginosa<\/jats:italic>and<jats:italic>S.\u00a0aureus<\/jats:italic>, even following penetration into the deeper corneal stromal layers.<\/jats:p>","DOI":"10.1111\/j.1755-3768.2012.02501.x","type":"journal-article","created":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T12:00:40Z","timestamp":1347451240000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>N<\/i>\u2010chlorotaurine and its analogues<i>N,N<\/i>\u2010dichloro\u20102,2\u2010dimethyltaurine and<i>N<\/i>\u2010monochloro\u20102,2\u2010dimethyltaurine are safe and effective bactericidal agents in<i>ex vivo<\/i>corneal 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