{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T02:09:23Z","timestamp":1768874963259,"version":"3.49.0"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,9,20]],"date-time":"2012-09-20T00:00:00Z","timestamp":1348099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p><jats:bold>Purpose:\u2002<\/jats:bold> Ranibizumab (Lucentis<jats:sup>\u00ae<\/jats:sup>) is a Fab\u2010Fragment of a recombinant, humanized, monoclonal VEGF (anti\u2010vascular endothelial growth factor) antibody. This study analyzed the ability of topical Ranibizumab to inhibit lymphangiogenesis in addition to hemangiogenesis after acute corneal inflammation <jats:italic>in vivo<\/jats:italic>. In addition, the effect of Ranibizumab on the proliferation of human lymphatic endothelial cells (LECs) and blood endothelial cells (BECs) <jats:italic>in vitro<\/jats:italic> was studied.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> The inhibitory effect of Ranibizumab on LECs and BECs was studied <jats:italic>in vitro<\/jats:italic> using a proliferation enzyme\u2010linked immunosorbent assay (ELISA) assay. To study the <jats:italic>in vivo<\/jats:italic> effects of Ranibizumab, the mouse model of suture induced inflammatory corneal neovascularization was used. Study mice received topical Ranibizumab as eye drops. After 1\u2003week excised corneas were stained with LYVE\u20101 and CD31. Hemangiogenesis and lymphangiogenesis were analyzed morphometrically by using a semiautomatic method based on the image analyzing program Cell^F.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> An antiproliferative effect of Ranibizumab was seen <jats:italic>in vitro<\/jats:italic> on both human BECs and LECs with a significance of p\u2003&lt;\u20030.0001 and p\u2003&lt;\u20030.0004, respectively. <jats:italic>In vivo<\/jats:italic> experiments showed that topical application of Ranibizumab significantly inhibits both hemangiogenesis (p\u2003=\u20030.0026) and lymphangiogenesis (p\u2003=\u20030.0026) in the cornea.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Ranibizumab is a potent inhibitor of inflammatory corneal hemangiogenesis and lymphangiogenesis <jats:italic>in vivo<\/jats:italic> with a direct inhibitory effect on both endothelial cell types <jats:italic>in vitro<\/jats:italic>. This study for the first time demonstrates an inhibitory effect of Ranibizumab on lymphatic vessels which could have a wider range of clinical applications.<\/jats:p>","DOI":"10.1111\/j.1755-3768.2012.02525.x","type":"journal-article","created":{"date-parts":[[2012,9,20]],"date-time":"2012-09-20T09:45:18Z","timestamp":1348134318000},"page":"143-148","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Topical Ranibizumab inhibits inflammatory corneal hem\u2010 and 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