{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T03:14:41Z","timestamp":1779160481533,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T00:00:00Z","timestamp":1333065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ Microbiol Rep"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    A proteomic approach was used to identify several proteins induced by cyanide in the alkaliphilic bacterium\n                    <jats:italic>Pseudomonas pseudoalcaligenes<\/jats:italic>\n                    CECT5344, two of them, NitB and NitG, encoded by genes that belong to the\n                    <jats:italic>nit1C<\/jats:italic>\n                    gene cluster. The predicted products of the\n                    <jats:italic>nit1C<\/jats:italic>\n                    gene cluster are a Fis\u2010like \u03c3\n                    <jats:sup>54<\/jats:sup>\n                    \u2010dependent transcriptional activator (NitA), a nitrilase (NitC), an\n                    <jats:italic>S<\/jats:italic>\n                    \u2010adenosylmethionine superfamily member (NitD), an\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acyltransferase superfamily member (NitE), a trifunctional polypeptide of the AIRS\/GARS family (NitF), an NADH\u2010dependent oxidoreductase (NitH) and two hypothetical proteins of unknown function (NitB and NitG). RT\u2010PCR analysis suggested that\n                    <jats:italic>nitBCDEFGH<\/jats:italic>\n                    genes were co\u2010transcribed, whereas the regulatory\n                    <jats:italic>nitA<\/jats:italic>\n                    gene was divergently transcribed. Real\u2010time RT\u2010PCR revealed that expression of the\n                    <jats:italic>nitBCDEFGH<\/jats:italic>\n                    genes was induced by cyanide and repressed by ammonium. The\n                    <jats:italic>P.\u2003pseudoalcaligenes<\/jats:italic>\n                    CECT5344\n                    <jats:italic>nit1C<\/jats:italic>\n                    gene cluster was found to be involved in assimilation of free and organic cyanides (nitriles) as deduced for the inability to grow with cyanides showed by the NitA, NitB and NitC mutant strains. The wild\u2010type strain CECT5344 showed a nitrilase activity that allows growth on cyanide or hydroxynitriles. The NitB and NitC mutants only presented low basal levels of nitrilase activity that were not enough to support growth on either free cyanide or aliphatic nitriles, suggesting that nitrilase NitC is specific and essential for cyanide and aliphatic nitriles assimilation.\n                  <\/jats:p>","DOI":"10.1111\/j.1758-2229.2012.00337.x","type":"journal-article","created":{"date-parts":[[2012,4,3]],"date-time":"2012-04-03T09:11:34Z","timestamp":1333444294000},"page":"326-334","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["The\n                    <i>nit1C<\/i>\n                    gene cluster of\n                    <i>Pseudomonas pseudoalcaligenes<\/i>\n                    CECT5344 involved in assimilation of nitriles is essential for growth on cyanide"],"prefix":"10.1111","volume":"4","author":[{"given":"Jessica","family":"Estepa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor M.","family":"Luque\u2010Almagro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Manso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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