{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T10:06:06Z","timestamp":1749031566083},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T00:00:00Z","timestamp":1169510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>A PCR assay targeting the metalloprotease gene (<jats:italic>mprA<\/jats:italic>) of <jats:italic>Burkholderia pseudomallei<\/jats:italic> was developed for the specific detection of this organism in pure cultures and clinical samples. All other closely related organisms including <jats:italic>B.\u00a0mallei<\/jats:italic> the causative agent of glanders, and <jats:italic>B.\u00a0thailandensis<\/jats:italic> tested negative. <jats:italic>Burkholderia pseudomallei<\/jats:italic> DNA was successfully amplified from paraffin\u2010embedded lung tissue of a camel with a generalized <jats:italic>B.\u00a0pseudomallei<\/jats:italic> infection. The developed PCR assay can be used as a simple tool for the specific and sensitive detection of <jats:italic>B.\u00a0pseudomallei<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1863-2378.2007.01008.x","type":"journal-article","created":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T15:53:20Z","timestamp":1169567600000},"page":"44-50","source":"Crossref","is-referenced-by-count":16,"title":["Development and Clinical Evaluation of A PCR Assay Targeting the Metalloprotease Gene (<i>mprA<\/i>) of <i>B.\u00a0pseudomallei<\/i>"],"prefix":"10.1111","volume":"54","author":[{"given":"H.","family":"Neubauer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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