{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T08:42:08Z","timestamp":1697272928085},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2007,9,24]],"date-time":"2007-09-24T00:00:00Z","timestamp":1190592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Transmissible spongiform encephalopathies, also termed prion diseases, are fatal neurodegenerative disorders that affect both humans and animals, which are characterized by presences of protease\u2010resistance disease\u2010associated prion protein (PrP<jats:sup>Sc<\/jats:sup>) in brains. In the present study, we optimized the Western blot assay for PrP<jats:sup>Sc<\/jats:sup> with a precipitation procedure of streptomycin sulphate. After incubated with suitable amount of streptomycin sulphate, the detective sensitivity for PrP<jats:sup>Sc<\/jats:sup> was remarkably improved. The precipitation of PrP<jats:sup>Sc<\/jats:sup> was obviously influenced by pH value in the solution. Employs of PrP<jats:sup>Sc<\/jats:sup> stock sample into various mimic specimens, including normal hamster brain homogenate, human cerebrospinal fluid and urine, demonstrated that streptomycin precipitation markedly increased the detective sensitivity of PrP<jats:sup>Sc<\/jats:sup>, regardless in low concentration or in large volume. In addition, the PrP<jats:sup>Sc<\/jats:sup> from a human brain tissue of familiar Creutzfeldt\u2013Jakob disease (fCJD) was efficiently precipitated with streptomycin sulphate. As a sensitive, specific, rapid and flexible protocol for PrP<jats:sup>Sc<\/jats:sup>, the protocol in this study has the potential, alone or combined with other techniques, to detect low levels of PrP<jats:sup>Sc<\/jats:sup> in the specimens not only from central nerve system, but also from peripheral organs or fluids.<\/jats:p>","DOI":"10.1111\/j.1863-2378.2007.01062.x","type":"journal-article","created":{"date-parts":[[2007,9,24]],"date-time":"2007-09-24T13:47:11Z","timestamp":1190641631000},"page":"328-336","source":"Crossref","is-referenced-by-count":7,"title":["Sensitive Detection of PrP<sup>Sc<\/sup> by Western Blot Assay Based on Streptomycin Sulphate Precipitation"],"prefix":"10.1111","volume":"54","author":[{"given":"C.\u2010F.","family":"Dong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.\u2010X.","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u2010M.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.\u2010F.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Shan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.\u2010J.","family":"Lei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.\u2010Y.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.\u2010P.","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,9,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.5C6895.2006"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/39057"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(99)00077-5"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200390107"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)05128-4"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/383685a0"},{"key":"e_1_2_6_8_1","first-page":"8","article-title":"Dynamic analyses of PrP and PrP(Sc) in brain tissues of golden hamsters infected with scrapie strain 263K revealed various PrP forms","volume":"17","author":"Gao J. 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