{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T15:02:50Z","timestamp":1776351770163,"version":"3.51.2"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2007,9,24]],"date-time":"2007-09-24T00:00:00Z","timestamp":1190592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium (<jats:italic>S<\/jats:italic>. Typhimurium) is a facultative intracellular bacterium which can infect and colonize pigs. After contact with enterocytes and macrophages, <jats:italic>S<\/jats:italic>. Typhimurium induces production of cytokines thus triggering the innate immune response. In this study we evaluated the cytokine response of two porcine cell lines, IPI\u20102I and 3D4\/31, of epithelial or macrophage origins, respectively, to the wild\u2010type <jats:italic>S<\/jats:italic>. Typhimurium and its <jats:italic>hilA<\/jats:italic> and <jats:italic>ssrA<\/jats:italic> mutants. We observed that the 3D4\/31 cell line essentially did not respond to <jats:italic>S<\/jats:italic>. Typhimurium infection when a medium with foetal calf serum was used. However when the 3D4 cell line was incubated overnight in the presence of porcine serum, it efficiently responded to the wild\u2010type strain and the <jats:italic>ssrA<\/jats:italic> mutant but not to the noninvasive <jats:italic>hilA<\/jats:italic> mutant as measured by mRNA quantification of TNF\u2010\u03b1, IL\u20108 and GM\u2010CSF by the real\u2010time RT\u2010PCR. In IPI\u20102I, all the cytokines were also induced by the wild\u2010type <jats:italic>S<\/jats:italic>. Typhimurium and the <jats:italic>ssrA<\/jats:italic> mutant although the induction of TNF\u2010\u03b1 was lower than that induced by the wild\u2010type strain. The <jats:italic>hilA<\/jats:italic> mutant was unable to induce any of the cytokines tested. The <jats:italic>ssrA<\/jats:italic> mutant can therefore be considered as more suitable for further vaccine development as the stimulation of innate immune response is important for animal protection against a challenge with virulent strains.<\/jats:p>","DOI":"10.1111\/j.1863-2378.2007.01064.x","type":"journal-article","created":{"date-parts":[[2007,9,24]],"date-time":"2007-09-24T13:47:11Z","timestamp":1190641631000},"page":"286-293","source":"Crossref","is-referenced-by-count":27,"title":["Cytokine Response of Porcine Cell Lines to <i>Salmonella enterica<\/i> serovar Typhimurium and its <i>hilA<\/i> and <i>ssrA<\/i> mutants"],"prefix":"10.1111","volume":"54","author":[{"given":"J.","family":"Volf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Boyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Faldyna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Pavlova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Navratilova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Rychlik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,9,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-145-9-2431"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.10.018"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.09.008"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2004.00588.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02192.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"5238","DOI":"10.1128\/iai.65.12.5238-5243.1997","article-title":"Pathogenicity and protective effect of rough mutants of Salmonella species in germ\u2010free piglets","volume":"65","author":"Dlabac V.","year":"1997","journal-title":"Infect. 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