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After enrichment (mTSB with novobiocin), samples were screened by real\u2010time PCR first for <jats:italic>stx<\/jats:italic> and if positive, tested for the top\u2010five Shiga toxin\u2010producing <jats:italic>Escherichia coli<\/jats:italic> (STEC) serogroups using PCR assays targeting genes specific for serogroups O26, O103, O111, O145 and O157. Of 563 samples with available results, 74.1% tested positive for <jats:italic>stx<\/jats:italic> genes. Amongst them, the serogroups O145, O103, O26, O157 and O111 were detected in 41.9%, 25.9%, 23.9%, 7.8% and 0.8%, respectively. From 95 O26, 166 O145 and 30 O157 PCR\u2010positive samples, 17 O26, 28 O145 and 12 O157 strains were isolated by colony hybridization after immunomagnetic separation. The 17 O26 strains were <jats:italic>eae<\/jats:italic>\u2010positive, but only nine strains harboured <jats:italic>stx<\/jats:italic> (eight possessing <jats:italic>stx<\/jats:italic>1 and one <jats:italic>stx<\/jats:italic>2). Of the 28 O145 strains, ten were <jats:italic>eae<\/jats:italic>\u2010positive including four harbouring <jats:italic>stx<\/jats:italic>1 or <jats:italic>stx<\/jats:italic>2, whereas 18 were negative for <jats:italic>stx<\/jats:italic> and <jats:italic>eae<\/jats:italic>. Five of the 12 O157 strains harboured <jats:italic>stx<\/jats:italic>2 and <jats:italic>eae<\/jats:italic>, did not ferment sorbitol, and were identified as STEC O157:H7\/H<jats:sup>\u2212<\/jats:sup>. The other seven O157 strains were negative for <jats:italic>stx<\/jats:italic> and <jats:italic>eae<\/jats:italic> or positive only for <jats:italic>eae<\/jats:italic>. Shiga toxin genes and the top\u2010five STEC serogroups were frequently found in young Swiss cattle at slaughter, but success rates for strain isolation were low and only few strains showed a virulence pattern of human pathogenic STEC.<\/jats:p>","DOI":"10.1111\/j.1863-2378.2012.01468.x","type":"journal-article","created":{"date-parts":[[2012,2,21]],"date-time":"2012-02-21T07:53:12Z","timestamp":1329810792000},"page":"408-415","source":"Crossref","is-referenced-by-count":24,"title":["Application of a Real\u2010Time PCR\u2010Based System for Monitoring of O26, O103, O111, O145 and O157 Shiga Toxin\u2010Producing <i>Escherichia coli<\/i> in Cattle at 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