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Overall, 143 (22%) samples were<jats:italic>Cryptosporidium<\/jats:italic>positive, while three (0.5%) were positive for<jats:italic>Giardia<\/jats:italic>. Successful genotyping of 25<jats:italic>Cryptosporidium<\/jats:italic>isolates by sequence analysis of the HSP70 gene revealed that 28 and 72% were<jats:italic>C.\u00a0hominis<\/jats:italic>and<jats:italic>C.\u00a0parvum,<\/jats:italic>respectively.<jats:italic>Cryptosporidium<\/jats:italic>isolates from humans and previously genotyped<jats:italic>C.\u00a0parvum<\/jats:italic>from beef cattle were subtyped by sequence analysis of the GP60 gene. Subtyping identified three subtypes belonging to the family IIa. All three subtypes IIaA16G2RI (55%), IIaA16G3RI (22%) and IIaA15G2RI (22%) were found in the animal isolates, while two of the subtypes found in the animals, IIaA16G2RI (80%) and IIaA15G2RI (20%), were also identified in the human isolates.<jats:italic>Cryptosporidium<\/jats:italic>infection in humans peaked in April\u2013June. Molecular epidemiological analysis of the human data showed a<jats:italic>C.\u00a0parvum<\/jats:italic>peak in the spring and a relatively smaller peak for<jats:italic>C.\u00a0hominis<\/jats:italic>in July\u2013September. The majority (57%) of human<jats:italic>Cryptosporidium<\/jats:italic>isolates were found in children between 5 and 10\u2003years of age. All three<jats:italic>Giardia<\/jats:italic>isolates were identified as<jats:italic>G.\u00a0duodenalis<\/jats:italic>assemblage A. The overall<jats:italic>Cryptosporidium<\/jats:italic>prevalence in our human samples was high relative to other studies, but because the samples were submitted to a hospital diagnostic laboratory, the results may not be representative of the general population. Further, the presence of the same zoonotic<jats:italic>C.\u00a0parvum<\/jats:italic>subtypes in cattle and human isolates implies that transmission is largely zoonotic and cattle may be a source of sporadic human infections on PEI. The presence of<jats:italic>Giardia<\/jats:italic>in people on PEI is rare, and the assemblage A found in humans might originate from humans, livestock or other domestic or wild animals.<\/jats:p>","DOI":"10.1111\/j.1863-2378.2012.01474.x","type":"journal-article","created":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T10:06:28Z","timestamp":1331028388000},"page":"424-433","source":"Crossref","is-referenced-by-count":30,"title":["Molecular Epidemiology of<i>Cryptosporidium<\/i>and<i>Giardia<\/i>in Humans on Prince Edward Island, Canada: Evidence of Zoonotic Transmission From Cattle"],"prefix":"10.1111","volume":"59","author":[{"given":"E.","family":"Budu\u2010Amoako","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. J.","family":"Greenwood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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