{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T09:41:18Z","timestamp":1793180478468,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,5,26]],"date-time":"2007-05-26T00:00:00Z","timestamp":1180137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold> The cytoprotective effects of six novel synthetic prostaglandin A<jats:sub>2<\/jats:sub> analogs against carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) as a toxic agent were studied with isolated rat liver hepatocytes <jats:italic>in vitro<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> It was found that hepatocytes treatment with CCl<jats:sub>4<\/jats:sub> induced: (i) a significant increase of lactic dehydrogenase (LDH) release from cytoplasm; (ii) leakage of glutamate dehydrogenase (GDH) and acid phosphatase from mitochondria and lysosomes, respectively; (iii) 10\u2010fold increase of trien conjugates formation; and (iv) a reduction of free SH\u2010groups by 50%. Prostanoids U\u201026, U\u20109 and U\u201034 decreased cytotoxic index of CCl<jats:sub>4<\/jats:sub> on average by 1.5\u20132.0 times and were more effective than PGI<jats:sub>2<\/jats:sub>, the well\u2010known hepatoprotector of prostanoids type. The protective action of the prostanoids was not a cAMP\u2010 or Ca<jats:sup>2+<\/jats:sup>\u2010dependent process. However, prostanoids U\u201026, U\u20109 and U\u201034 normalized intracellular content of SH\u2010groups, reduced trien conjugates formation by 60\u201380% and strongly prevented enzyme leakage through cellular membranes. They were also able to inhibit CCl<jats:sub>4<\/jats:sub> effects via decreasing cytochrome P<jats:sub>450<\/jats:sub>2E1 activity.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The results obtained demonstrate that prostanoids provide cytoprotective effects on liver hepatocytes through the prevention of lipid peroxidation of the plasma and the cellular membranes and maintenance of their barrier function.<\/jats:p>","DOI":"10.1111\/j.1872-034x.2007.00066.x","type":"journal-article","created":{"date-parts":[[2007,5,26]],"date-time":"2007-05-26T11:06:07Z","timestamp":1180177567000},"page":"416-424","source":"Crossref","is-referenced-by-count":6,"title":["Hepatoprotective action of prostaglandin A<sub>2<\/sub> analogs under CCl<sub>4<\/sub>\u2010induced liver injury <i>in vitro<\/i>"],"prefix":"10.1111","volume":"37","author":[{"given":"Aksana I.","family":"Hubich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey Y.","family":"Bondar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsiana U.","family":"Kastsiuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uladimir A.","family":"Kastsiuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fyodar A.","family":"Lakhvich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail V.","family":"Sholukh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,5,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.294.5548.1871"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297906030011"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-56553-3_1"},{"key":"e_1_2_6_5_2","first-page":"388","article-title":"The role of some prostaglandin analogues in experimental intoxication produced by carbon tetrachloride in rats.","volume":"107","author":"Filip C","year":"2003","journal-title":"Rev Med Chir Soc Med Nat"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(93)90067-N"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365528809090149"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2003.02688.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1128\/AAC.40.2.367","article-title":"Inhibition of poliovirus replication by prostaglandins A and J in human cells.","volume":"40","author":"Conti C","year":"1996","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/med.1006"},{"key":"e_1_2_6_11_2","first-page":"1854","article-title":"Synthesis of aminoprostanoids of A\u2010 and J\u2010types.","volume":"28","author":"Lis LG","year":"1992","journal-title":"J Org Chem (Moscow)"},{"key":"e_1_2_6_12_2","first-page":"2394","article-title":"Synthesis of 10\u2010methyl\u2010derivates of bis\u201012a,20\u2010homoprostaglandins by the convergent plan.","volume":"29","author":"Lis LG","year":"1993","journal-title":"J Org Chem (Moscow)"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.43.3.506"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.12.196"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840060108"},{"key":"e_1_2_6_16_2","first-page":"387","article-title":"Normberiechte von alpha\u2010HBDH, LDH, AP and LAP bei Messung mit substant\u2010optimierten Testabsatzen.","volume":"26","author":"Weisshaar D","year":"1975","journal-title":"Med Welt"},{"key":"e_1_2_6_17_2","first-page":"495","volume-title":"Methods of Enzymatic Analysis","author":"Bergmeyer HU","year":"1974"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-091302-2.50028-1"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(78)90025-X"},{"key":"e_1_2_6_20_2","first-page":"125","article-title":"Determination of dien conjugates by spectrophotometry.","volume":"30","author":"Kostyuk VA","year":"1984","journal-title":"Voprosi Med Chem"},{"key":"e_1_2_6_21_2","first-page":"313","article-title":"Statins rise cytoplasmic calcium level in cultured endothelial cells.","volume":"56","author":"Lorkowska B","year":"2004","journal-title":"Pol J Pharmacol"},{"key":"e_1_2_6_22_2","first-page":"1952","article-title":"Effect of serotonin\u20103 receptor antagonists on cytochrome P450 activities in human liver microsomes.","volume":"26","author":"Niwa T","year":"2006","journal-title":"Biol Pharm Bull"},{"key":"e_1_2_6_23_2","doi-asserted-by":"crossref","unstructured":"A PetersonGL.Determination of total protein. 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