{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T19:48:37Z","timestamp":1723405717532},"reference-count":56,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2007,5,22]],"date-time":"2007-05-22T00:00:00Z","timestamp":1179792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold> Fas membrane\u2010associated polypeptide antigen is a receptor molecule responsible for apoptosis\u2010mediated signals. In animal models of acute viral hepatitis, apoptosis of hepatocytes is mediated by Fas\u2010death receptors; therefore, the aim of this study was to evaluate the effect of interferon (IFN)\u2010\u03b1 on apoptotic markers and nuclease activity against different coding and non\u2010coding single and double stranded RNAs during Fas\u2010induced liver apoptosis.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> An <jats:italic>in vivo<\/jats:italic> experiment was performed with simultaneous administration of anti\u2010Fas (CD95) antibodies and IFN\u2010\u03b1, and an <jats:italic>in vitro<\/jats:italic> experiment was performed in hepatocyte cultures treated with anti\u2010Fas antibodies and IFN\u2010\u03b1.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Detection of apoptosis using Annexin V\u2010FITC\/propidium iodide, Bcl\u20102 and Bax expression in hepatocyte cultures confirmed the appearance of early apoptotic events and progression toward late apoptosis after anti\u2010Fas antibody treatment. IFN\u2010\u03b1 had a tendency to retard the apoptosis process in Fas\u2010induced apoptosis by increasing the number of viable cells and decreasing the number of cells in late apoptosis, by increasing the percentage of Bcl\u20102 positive cells, by decreasing the percentage of Bax positive cells, and by decreasing the nuclease activity compared to the anti\u2010Fas antibody treated group. Total DNA and RNA concentration was much reduced in the Fas group and DNA fragmentation assay provided evidence for increased DNA degradation. Enhanced nuclease activity against DNA, rRNA, poly(A), poly(C), poly(U), poly(I:C), and poly(A:U) was manifested in the anti\u2010Fas antibody treated group, except for the inhibitory\u2010bound alkaline RNase.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The results demonstrate that the RNA\u2010degrading pathway in Fas\u2010induced apoptosis can accelerate the liberation of the latent enzyme from the inhibitor complex. IFN\u2010\u03b1 prevented enormous, Fas\u2010ligand induced degradation of all the substrates used in this experimental study, most probably due to similarities in the signal transduction pathways. Investigations of death receptor\u2010induced apoptosis may lead to novel treatment combinations for patients with acute or chronic liver diseases.<\/jats:p>","DOI":"10.1111\/j.1872-034x.2007.00090.x","type":"journal-article","created":{"date-parts":[[2007,5,23]],"date-time":"2007-05-23T04:55:05Z","timestamp":1179896105000},"page":"637-646","source":"Crossref","is-referenced-by-count":6,"title":["Protective effect of interferon\u2010\u03b1 on the DNA\u2010 and RNA\u2010degrading pathway in anti\u2010Fas\u2010antibody induced 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