{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T23:52:58Z","timestamp":1776556378440,"version":"3.51.2"},"reference-count":67,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,8,18]],"date-time":"2008-08-18T00:00:00Z","timestamp":1219017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold>N\u2010acetylcysteine can inhibit the formation of intracellular reactive oxygen intermediates. Cellular redox state plays a role in regulating the secretion of matrix metalloproteinase\u20102. We investigated the effects of N\u2010acetylcysteine on the expression of matrix metalloproteinase\u20102 and tissue inhibitor of matrix metalloproteinase\u20102.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>Bile duct ligated rats were used as a model of hepatic fibrosis. We compared the level of gene expression (using real\u2010time reverse transcription polymerase chain reaction [RT\u2013PCR]), liver function parameters, hepatic reactive oxygen production, lipid peroxidation and glutathione state in experimental groups.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>N\u2010acetylcysteine treatment significantly improved liver function parameters including the plasma levels of aspartate aminotransferase, alkaline phosphatase, gamma\u2010glutamyl transpeptidase and bilirubin. In addition, significant improvement of glutathione state and reactive oxygen production were observed. Hepatic lipid peroxidation was reversed by N\u2010acetylcysteine treatment. Although N\u2010acetylcysteine treatment did not completely normalize the increased matrix metalloproteinase\u20102 expression, it significantly decreased its level by 65%. N\u2010acetylcysteine treatment also significantly decreased matrix metalloproteinase\u20102 activity and normalized tissue inhibitor of matrix metalloproteinase\u20102 expression.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold>Collectively, N\u2010acetylcysteine showed inhibition of matrix metalloproteinase\u20102 expression and activity. In addition, administration of N\u2010acetylcysteine was associated with downregulation of the expression of tissue inhibitor of matrix metalloproteinase\u20102 and amelioration of oxidative stress in the liver of bile duct ligated rats.<\/jats:p>","DOI":"10.1111\/j.1872-034x.2008.00393.x","type":"journal-article","created":{"date-parts":[[2008,8,18]],"date-time":"2008-08-18T14:49:17Z","timestamp":1219070957000},"page":"1252-1263","source":"Crossref","is-referenced-by-count":7,"title":["The effects of N\u2010acetylcysteine on the expression of matrix metalloproteinase\u20102 and tissue inhibitor of matrix metalloproteinase\u20102 in hepatic fibrosis in bile duct ligated rats"],"prefix":"10.1111","volume":"38","author":[{"given":"Arezou","family":"Rezaei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sussan 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