{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T06:12:49Z","timestamp":1697436769153},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,11,13]],"date-time":"2008-11-13T00:00:00Z","timestamp":1226534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold> We investigated the relationship between the magnitude of comprehensive hepatitis C virus (HCV)\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell responses and the clinical course of acute HCV infection.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Six consecutive patients with acute HCV infection were studied. Analysis of HCV\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell responses was performed using an interferon\u2010\u03b3\u2010based enzyme\u2010linked immunospot assay using peripheral CD8<jats:sup>+<\/jats:sup> T\u2010cells, monocytes and 297 20\u2010mer synthetic peptides overlapping by 10 residues and spanning the entire HCV sequence of genotype 1b.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Five patients presented detectable HCV\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell responses against a single and different peptide, whereas 1 patient showed responses against three different peptides. Neither the magnitude of HCV\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell responses nor the severity of hepatitis predicts the outcome of acute hepatitis. The maximum number of HCV\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cells correlated with maximum serum alanine aminotransferase level during the course (<jats:italic>r<\/jats:italic>\u2003=\u20030.841, <jats:italic>P\u2003<\/jats:italic>=\u20030.036).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> HCV\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell responses were detectable in all 6 patients with acute HCV infection, and 6 novel HCV\u2010specific CTL epitopes were identified. Acute HCV infection can resolve with detectable HCV\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell responses, but without development of antibody against HCV.<\/jats:p>","DOI":"10.1111\/j.1872-034x.2008.00459.x","type":"journal-article","created":{"date-parts":[[2008,11,13]],"date-time":"2008-11-13T08:03:57Z","timestamp":1226563437000},"page":"256-265","source":"Crossref","is-referenced-by-count":5,"title":["Magnitude of CD8<sup>+<\/sup> T\u2010cell responses against hepatitis C virus and severity of hepatitis do not necessarily determine outcomes in acute hepatitis C virus 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