{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:46:57Z","timestamp":1790282817738,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,4,26]],"date-time":"2011-04-26T00:00:00Z","timestamp":1303776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold> To study the effect of retinoid X receptor\u2010\u03b1 (RXR\u2010\u03b1) expression on rat hepatic fibrosis.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Rat hepatic fibrosis was induced by CCl<jats:sub>4<\/jats:sub>, and the rats were randomly divided into an early\u2010phase hepatic fibrosis group (2\u2003weeks) and a sustained hepatic fibrosis group (8\u2003weeks). They were then divided into four groups (normal control, hepatic fibrosis, negative control and RXR\u2010\u03b1 groups). A recombinant lentiviral expression vector carrying the rat RXR\u2010\u03b1 gene was injected into the rats to induce RXR\u2010\u03b1 expression by intraportal infusion, hepatic tissue pathological examination was performed, and hydroxyproline content was detected. Hepatic stellate cells (HSC) were cultured <jats:italic>in vitro<\/jats:italic>, an RXR\u2010\u03b1 lentivirus vector was used to activate HSC, and 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide (MTT) activation was assayed to detect HSC proliferation.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>  <jats:italic>In vivo<\/jats:italic> experiments indicated that in the sustained hepatic fibrosis group, there were significant differences in the hydroxyproline content, and expression of RXR\u2010\u03b1, \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) and type I collagen (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01). However, in the early\u2010phase hepatic fibrosis group, hydroxyproline content and the protein level of RXR\u2010\u03b1 showed no significant difference compared with the normal control group (<jats:italic>P<\/jats:italic>\u2003&gt;\u20030.05). <jats:italic>In vitro<\/jats:italic> studies revealed that expression of RXR\u2010\u03b1 significantly inhibited expression of \u03b1\u2010SMA and type I collagen in activated HSC (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01), as well as HSC proliferation (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01).<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The increased RXR\u2010\u03b1 gene expression inhibited HSC activation and proliferation and the degree of hepatic fibrosis.<\/jats:p>","DOI":"10.1111\/j.1872-034x.2011.00794.x","type":"journal-article","created":{"date-parts":[[2011,4,26]],"date-time":"2011-04-26T09:20:36Z","timestamp":1303809636000},"page":"475-483","source":"Crossref","is-referenced-by-count":18,"title":["Effect of the regulation of retinoid X receptor\u2010\u03b1 gene expression on rat hepatic 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