{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T04:50:44Z","timestamp":1749099044345},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold> In Japan, the etiology of 10\u201320% of cases of acute hepatitis remains unclarified. This study was conducted to verify the agent causing non\u2010A\u2013E hepatitis.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Serum samples from 500 blood donors with elevated alanine aminotransferase (ALT) levels were screened by polymerase chain reaction using primers constructed from conserved areas of RNA virus helicase. The sequence obtained was investigated for viral properties.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Four blood samples were found to contain a novel DNA sequence of 9496\u2003bp, which was designated KIs\u2010V. KIs\u2010V was sensitive to the restriction enzyme <jats:italic>Sal<\/jats:italic>I and <jats:italic>Bst<\/jats:italic>XI. Rolling\u2010circle amplification produced an excessive amount of KIs\u2010V DNA. In sucrose density gradient ultracentrifugation, KIs\u2010V banded at a 1.158\u2010g\/cm<jats:sup>3<\/jats:sup> density. Detergent treatment increased the density of KIs\u2010V. There was no KIs\u2010V DNA amplification from human leukocyte DNA. Serial filtration suggested that KIs\u2010V was included in a 30\u201350\u2010nm size particle. <jats:italic>In silico<\/jats:italic> analysis revealed that KIs\u2010V contained 13 potential genes, none of which showed homology to any viral proteins reported. One gene showed similarity to a DNA polymerase domain. Strong signals for transcription initiation and a CpG island were identified. The nucleotide composition of KIs\u2010V showed a characteristic feature of circular DNA genomes that contain a replication origin and a terminus. In a preliminary study, KIs\u2010V was frequently identified among hepatitis E virus antibody positive individuals with elevated ALT levels.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> A new sequence KIs\u2010V was isolated from blood donors with elevated ALT levels. It was suggested that KIs\u2010V is a double\u2010stranded circular DNA genome derived from a novel category of enveloped viruses.<\/jats:p>","DOI":"10.1111\/j.1872-034x.2011.00848.x","type":"journal-article","created":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T09:09:05Z","timestamp":1309511345000},"page":"971-981","source":"Crossref","is-referenced-by-count":5,"title":["Novel DNA sequence isolated from blood donors with high transaminase 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