{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T08:14:34Z","timestamp":1697444074478},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,1,11]],"date-time":"2012-01-11T00:00:00Z","timestamp":1326240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold> Several mouse models of inflammatory cholangiopathies exist, including biliary atresia, primary biliary cirrhosis, autoimmune hepatitis, and primary sclerosing cholangitis. In an ongoing effort to identify the target antigens of both infiltrating autoreactive T cells and serum autoantibodies, we aimed to generate a cholangiocyte\u2010derived cDNA library capable of expressing a wide variety of proteins.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> mRNA was isolated from a normal mouse cholangiocyte cell line and reverse transcribed into cDNA. After initial cloning of the cDNA into a transfer vector (pDONR222), the entire library was shuttled into an <jats:italic>Escherichia\u2003coli<\/jats:italic> expression vector (pDEST160).<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The library contains 2.3\u2003\u00d7\u200310<jats:sup>6<\/jats:sup> independent clones and expresses proteins up to 100\u2003kD in molecular weight. Using a variety of techniques, including western blot analysis, mass spectrometry of individual clones, and direct DNA sequencing of plasmids, a number of both ubiquitously expressed and cholangiocyte\u2010specific proteins (e.g. cytokeratin 19) have been identified within.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> A comprehensive mouse cholangiocyte cDNA expression library has been generated and is available for use as a source of multiple cholangiocyte\u2010specific antigens for immunological studies. The library can be used to screen for specificity of T cell lines or hybridomas. Furthermore, this library has potential uses in SEREX analysis of autoantibody reactivity. The cholangiocyte\u2010specific cDNA library is a powerful tool for the identification of target antigens in murine inflammatory cholangiopathies and is available as a shared resource.<\/jats:p>","DOI":"10.1111\/j.1872-034x.2011.00951.x","type":"journal-article","created":{"date-parts":[[2012,1,12]],"date-time":"2012-01-12T02:31:49Z","timestamp":1326335509000},"page":"502-507","source":"Crossref","is-referenced-by-count":0,"title":["Generation of a cholangiocyte\u2010specific cDNA expression library for the identification of B and T cell autoantigens in murine biliary disease"],"prefix":"10.1111","volume":"42","author":[{"given":"Rebecca M.","family":"Tucker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cara L.","family":"Mack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004280050077"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.04.009"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000282072"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364248"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.27013"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21366"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.07.042"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2005.01.012"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.04.031"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-9972(03)00047-8"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.07396.x"},{"key":"e_1_2_6_13_2","first-page":"319","article-title":"Potential target antigens for immunotherapy identified by serological expression cloning (SEREX)","volume":"360","author":"Jager D","year":"2007","journal-title":"Methods Mol Biol"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2005.02197.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/12465"},{"key":"e_1_2_6_16_2","first-page":"421","volume-title":"The Liver; 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