{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T06:34:23Z","timestamp":1768718063430,"version":"3.49.0"},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,5,17]],"date-time":"2012-05-17T00:00:00Z","timestamp":1337212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold> Splenectomy or partial splenic embolism has been reported to improve liver function in patients with hypersplenism and liver dysfunction. The aim of this study was to investigate the mechanism of improvement after splenectomy.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Liver cirrhosis was induced by bile duct ligation (BDL). Rats underwent sham operation, splenectomy (Sp group), BDL, or BDL plus splenectomy (BDL\u2003+\u2003Sp group), and were subjected to experiments at 2\u2003weeks after the operation. Portal venous pressure (PVP) and hepatic tissue blood flow (HTBF) were measured in each group. The plasma concentration of endothelin\u20101 (ET\u20101) and endothelial nitric oxide synthase (eNOS), RhoA and Rho\u2010kinase expressions were studied.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> There were significant differences in PVP (17.9\u2003\u00b1\u20030.91 vs 23.3\u2003\u00b1\u20033.91\u2003cmH<jats:sub>2<\/jats:sub>O; <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01) and HTBF (16.6\u2003\u00b1\u20031.72 vs 13.3\u2003\u00b1\u20031.82\u2003mL\/min; <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01) between the BDL\u2003+\u2003Sp and BDL groups. In the liver of BDL rats, eNOS phosphorylation and NOx levels were decreased, accompanied by RhoA activation compared with the BDL\u2003+\u2003Sp group. Splenectomy decreased serum ET\u20101 levels, RhoA activation and consequently increased eNOS phosphorylation.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> ET\u20101 derived from the spleen might increase intrahepatic resistance by downregulating Rho signaling in liver cirrhosis. Splenectomy for splenomegaly in liver cirrhosis might partially improve liver function by enhancing intrahepatic microcirculation.<\/jats:p>","DOI":"10.1111\/j.1872-034x.2012.01021.x","type":"journal-article","created":{"date-parts":[[2012,4,20]],"date-time":"2012-04-20T08:28:30Z","timestamp":1334910510000},"page":"1039-1047","source":"Crossref","is-referenced-by-count":17,"title":["Endothelin\u20101 derived from spleen\u2010activated Rho\u2010kinase pathway in rats with secondary biliary cirrhosis"],"prefix":"10.1111","volume":"42","author":[{"given":"Hideo","family":"Uehara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiko","family":"Akahoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohumi","family":"Kawanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naotaka","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Nagao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morimasa","family":"Tomikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akinobu","family":"Taketomi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Shirabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Hashizume","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Maehara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,5,17]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199306243282508"},{"key":"e_1_2_5_3_2","first-page":"G618","article-title":"Evolution 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