{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:04:58Z","timestamp":1777392298381,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,6,5]],"date-time":"2012-06-05T00:00:00Z","timestamp":1338854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p><jats:bold>Aim:\u2002<\/jats:bold> Single nucleotide polymorphisms (SNP) around interferon (IFN)\u2010\u03bb3 have been associated with the response to pegylated IFN\u2010\u03b1 treatment for chronic hepatitis C. Specific quantification methods for IFN\u2010\u03bb3 are required to facilitate clinical and basic study.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Gene\u2010specific primers and probes for IFN\u2010\u03bb1, 2 and 3 were designed for real\u2010time detection PCR (RTD\u2013PCR). Dynamic range and specificity were examined using specific cDNA clones. Total RNA from hematopoietic and hepatocellular carcinoma cell lines was prepared for RTD\u2013PCR. Monoclonal antibodies were developed for the IFN\u2010\u03bb3\u2010specific immunoassays. The immunoassays were assessed by measuring IFN\u2010\u03bb3 in serum and plasma.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The RTD\u2013PCR had a broad detection range (10\u201310<jats:sup>7<\/jats:sup>\u2003copies\/assay) with high specificity (\u223c10<jats:sup>7<\/jats:sup>\u2010fold specificity). Distinct expression profiles were observed in several cell lines. Hematopoietic cell lines expressed high levels of IFN\u2010\u03bb compared with hepatocellular carcinoma cells, and Sendai virus infection induced strong expression of IFN\u2010\u03bb. The developed chemiluminescence enzyme immunoassays (CLEIA) detected 0.1\u2003pg\/mL of IFN\u2010\u03bb3 and showed a wide detection range of 0.1\u201310\u2003000\u2003pg\/mL with little or no cross\u2010reactivity to IFN\u2010\u03bb1 or IFN\u2010\u03bb2. IFN\u2010\u03bb3 could be detected in all the serum and plasma samples by CLEIA, with median concentrations of 0.92 and 0.86\u2003pg\/mL, respectively.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Our newly developed RTD\u2013PCR and CLEIA assays will be valuable tools for investigating the distribution and functions of IFN\u2010\u03bb3, which is predicted to be a marker for predicting outcome of therapy for hepatitis C or other virus diseases.<\/jats:p>","DOI":"10.1111\/j.1872-034x.2012.01032.x","type":"journal-article","created":{"date-parts":[[2012,4,17]],"date-time":"2012-04-17T13:37:05Z","timestamp":1334669825000},"page":"1089-1099","source":"Crossref","is-referenced-by-count":19,"title":["Development of specific and quantitative real\u2010time detection PCR and immunoassays for 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