{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T18:01:10Z","timestamp":1773338470805,"version":"3.50.1"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Bacterin-based canine Leptospira vaccines could present a challenge for the use of whole blood real-time polymerase chain reaction (PCR) as a diagnostic tool. Recent vaccination could induce positive results if the targeted DNA fragment is present within the vaccine and in the blood of the recently vaccinated dog.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The objective of this study was to assess whether 2 available 4-serovar vaccines induce a positive real-time PCR reaction in the blood of healthy recently vaccinated dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Twenty healthy dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This was a prospective study. Dogs were assigned to 1 of 2 vaccine groups. Both vaccines were culture-based and include Leptospira interrogans serovars Pomona, Canicola, and Icterohaemorrhagiae and Leptospira kirschneri serovar Grippotyphosa. Whole blood for real-time PCR and serum for the microscopic agglutination test (MAT) were collected prior to and 3 and 7\u00a0days after vaccination and weekly thereafter for 8\u00a0weeks. Two real-time PCR tests targeting 2 different genes were performed independently in a blinded fashion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both Leptospira vaccines produced positive real-time PCR reactions when assayed undiluted or diluted 1\u00a0:\u00a0100 in canine blood. However, blood samples drawn from all dogs at all time points after vaccination were negative on PCR. All dogs developed MAT titers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>Recent vaccination with 2 commercially available vaccines does not interfere with the use of real-time PCR for the identification of acute Leptospira infection in dogs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/j.1939-1676.2011.00852.x","type":"journal-article","created":{"date-parts":[[2011,12,19]],"date-time":"2011-12-19T09:59:55Z","timestamp":1324288795000},"page":"149-152","source":"Crossref","is-referenced-by-count":34,"title":["Effects of Recent\n                    <i>Leptospira<\/i>\n                    Vaccination on Whole Blood Real-Time PCR Testing in Healthy Client-Owned Dogs"],"prefix":"10.1093","volume":"26","author":[{"given":"J.N.","family":"Midence","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Cornell University Department of Clinical Sciences, , Ithaca, NY"},{"name":"University of Pennsylvania School of Veterinary Medicine the Department of Clinical Studies, , PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.M.","family":"Leutenegger","sequence":"additional","affiliation":[{"name":"IDEXX Reference Laboratories , West Sacramento, CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.M.","family":"Chandler","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Cornell University Department of Clinical Sciences, , Ithaca, NY"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.E.","family":"Goldstein","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Cornell University Department of Clinical Sciences, , Ithaca, NY"},{"name":"The Animal Medical Center , New York, 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