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As competence increases in biofilms, a study was undertaken into the roles that these biofilm\u2010induced genes might play in transformation. Only five of the nine gene deletions had a significant effect on transformation efficiency. Deletion of the genes for recombinase A, <jats:italic>recA<\/jats:italic>, DNA processing protein, <jats:italic>dprA<\/jats:italic> and single\u2010stranded DNA\u2010binding protein, <jats:italic>ssbA<\/jats:italic>, produced results comparable with those from other bacteria, supporting the contention that these proteins have similar functions in <jats:italic>S.\u00a0mutans<\/jats:italic> competence. The uncharacterized genes <jats:italic>SMU.769<\/jats:italic> and <jats:italic>SMU.836<\/jats:italic> produced results in variance to deletion mutants of putative homologues in <jats:italic>S.\u00a0pneumoniae<\/jats:italic>. Deletion of <jats:italic>SMU.769<\/jats:italic> reduced chromosomal transformation 2.3\u2010fold. <jats:italic>SMU.769<\/jats:italic> belongs to a family of conserved genes induced by the competence\u2010stimulating peptide and which have no established function. In contrast, deletion of <jats:italic>SMU.836<\/jats:italic> reduced transformation of both plasmid and chromosomal DNA to &lt;3%. Homology searches suggested that Smu.836 belongs to a family of competence\u2010induced peptidoglycan hydrolases with a conserved enzyme domain and a species\u2010variable cell\u2010binding domain for which the best characterized member is the choline\u2010binding protein D, CbpD, of <jats:italic>S.\u00a0pneumoniae<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.2041-1014.2010.00589.x","type":"journal-article","created":{"date-parts":[[2010,10,29]],"date-time":"2010-10-29T12:45:10Z","timestamp":1288356310000},"page":"406-417","source":"Crossref","is-referenced-by-count":12,"title":["Deletion of competence\u2010induced genes over\u2010expressed in biofilms caused transformation deficiencies in <i>Streptococcus 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